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Product Info Summary
| SKU: | M01513-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-CD31 PECAM1 Rabbit Monoclonal Antibody
View all CD31/PECAM-1 Antibodies
SKU/Catalog Number
M01513-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD31 PECAM1 Rabbit Monoclonal Antibody catalog # M01513-1. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD31 PECAM1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01513-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CEE-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD31
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01513-1 is reactive to PECAM1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
43 kDa
Calculated molecular weight
82536 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01513-1 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:50
Positive Control
IHC: human kindey tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of CD31 expression in THP-1 cell lysates.
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Immunohistochemical analysis of paraffin-embedded human kindey, using CD31 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For PECAM1 (Source: Uniprot.org, NCBI)
Gene Name
PECAM1
Full Name
Platelet endothelial cell adhesion molecule
Weight
82536 MW
Alternative Names
Platelet endothelial cell adhesion molecule;PECAM-1;EndoCAM;GPIIA';PECA1;CD31;PECAM1; PECAM1 CD31, CD31/EndoCAM, GPIIA, PECA1, PECAM-1, endoCAM platelet and endothelial cell adhesion molecule 1 platelet endothelial cell adhesion molecule|CD31 |platelet endothelial cell adhesion molecule-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PECAM1, check out the PECAM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PECAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD31 PECAM1 Rabbit Monoclonal Antibody (M01513-1)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CD31 PECAM1 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-CD31 Rabbit Monoclonal antibody (M01513-1)?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
We do provide the blocking peptide for product anti-CD31 Rabbit Monoclonal antibody (M01513-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-26
Question
I was wanting to use your anti-CD31 Rabbit Monoclonal antibody for WB for human tendon of biceps brachii on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human tendon of biceps brachii identification?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
It shows on the product datasheet, M01513-1 anti-CD31 Rabbit Monoclonal antibody has been validated for Flow Cytometry, IP, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human tendon of biceps brachii in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-08
Question
Can you help my question with product M01513-1, anti-CD31 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01513-1 anti-CD31 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-26
Question
My boss were well pleased with the WB result of your anti-CD31 Rabbit Monoclonal antibody. However we have observed positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is PECAM1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
Based on literature, brain does express PECAM1. Generally PECAM1 expresses in cell membrane. Regarding which tissues have PECAM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2019-11-12
Question
Does M01513-1 anti-CD31 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Miller
Verified customer
Asked: 2019-06-03
Answer
It shows on the product datasheet, M01513-1 anti-CD31 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-03
Question
We have been able to see staining in human liver. Any tips? Is anti-CD31 Rabbit Monoclonal antibody supposed to stain liver positively?
M. Miller
Verified customer
Asked: 2019-04-18
Answer
From what I have seen in literature liver does express PECAM1. From what I have seen in Uniprot.org, PECAM1 is expressed in tendon of biceps brachii, lung, brain, platelet, liver, leukemic t-cell, among other tissues. Regarding which tissues have PECAM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2019-04-18
Question
Is this M01513-1 anti-CD31 Rabbit Monoclonal antibody reactive to the isotypes of PECAM1?
Verified Customer
Verified customer
Asked: 2018-12-25
Answer
The immunogen of M01513-1 anti-CD31 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD31. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-12-25
Question
I am interested in to test anti-CD31 Rabbit Monoclonal antibody M01513-1 on human tendon of biceps brachii for research purposes, then I may be interested in using anti-CD31 Rabbit Monoclonal antibody M01513-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-11-28
Answer
The products we sell, including anti-CD31 Rabbit Monoclonal antibody M01513-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-28
Question
We ordered your anti-CD31 Rabbit Monoclonal antibody for IHC on liver last year. I am using human, and We intend to use the antibody for WB next. I am interested in examining liver as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
W. Carter
Verified customer
Asked: 2018-06-20
Answer
I viewed the website and datasheets of our anti-CD31 Rabbit Monoclonal antibody and I see that M01513-1 has been validated on human in both IHC and WB. Thus M01513-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-06-20
Question
I see that the anti-CD31 Rabbit Monoclonal antibody M01513-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tendon of biceps brachii using anti-CD31 Rabbit Monoclonal antibody M01513-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-10-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-27
Question
We are currently using anti-CD31 Rabbit Monoclonal antibody M01513-1 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-18
Answer
The anti-CD31 Rabbit Monoclonal antibody (M01513-1) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-18
Question
Is there a BSA free version of anti-CD31 Rabbit Monoclonal antibody M01513-1 available?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD31 Rabbit Monoclonal antibody M01513-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-12
Question
Does anti-CD31 Rabbit Monoclonal antibody M01513-1 work for WB with tendon of biceps brachii?
G. Edwards
Verified customer
Asked: 2017-07-19
Answer
According to the expression profile of tendon of biceps brachii, PECAM1 is highly expressed in tendon of biceps brachii. So, it is likely that anti-CD31 Rabbit Monoclonal antibody M01513-1 will work for WB with tendon of biceps brachii.
Boster Scientific Support
Answered: 2017-07-19
Question
Please see the WB image, lot number and protocol we used for tendon of biceps brachii using anti-CD31 Rabbit Monoclonal antibody M01513-1. Please let me know if you require anything else.
B. Carter
Verified customer
Asked: 2016-06-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-06-08
