Product Info Summary
SKU: | PB9094 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-CD31/PECAM1 Antibody Picoband®
View all CD31/PECAM-1 Antibodies
SKU/Catalog Number
PB9094
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD31/PECAM1 Antibody Picoband® catalog # PB9094. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD31/PECAM1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9094)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Standard Protein
E.coli-derived human CD31 recombinant protein (Position: Q28-G382). Human CD31 shares 65% and 68% amino acid (aa) sequences identity with mouse and rat CD31, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9094 is reactive to PECAM1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90 kDa, 130 kDa
Calculated molecular weight
82536 MW
Background of CD31/PECAM-1
CD31 also known as Platelet endothelial cell adhesion molecule (PECAM-1), is a protein that in human is encoded by the PECAM1 gene. Encoded protein is a member of the immunoglobulin superfamily, CD31 is mapped to 17q23.3. CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is demonstrated that CD31 expression on human PBSCs may positively affect both neutrophil and platelet engraftment. Meanwhile, CD31 is involved in leukocyte migration and angiogenesis, which are key components of venous thrombus resolution.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9094 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, By Heat
Flow Cytometry(Fixed), 1-3 μg/1x106 cells
Positive Control
WB: human Jurkat whole cell, human Raji whole cell, human THP-1 whole cell
IHC: human hepatocellular carcinoma tissue, human placenta tissue
FCM: Jurkat cell
Validation Images & Assay Conditions
![pb9094 cd31 primary antibodies wb testing 1_1 pb9094 cd31 primary antibodies wb testing 1_1](https://www.bosterbio.com/media/catalog/product/p/b/pb9094-cd31-primary-antibodies-wb-testing-1_1.jpg)
Click image to see more details
Figure 1. Western blot analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PB9094).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human THP-1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31/PECAM1 antigen affinity purified polyclonal antibody (Catalog # PB9094) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD31/PECAM1 at approximately 90 kDa, 130 kDa. The expected band size for CD31/PECAM1 is at 82 kDa.
![pb9094 cd31 primary antibodies ihc testing 2_1 pb9094 cd31 primary antibodies ihc testing 2_1](https://www.bosterbio.com/media/catalog/product/p/b/pb9094-cd31-primary-antibodies-ihc-testing-2_1.jpg)
Click image to see more details
Figure 2. IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PB9094).
CD31/PECAM1 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/PECAM1 Antibody (PB9094) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![pb9094 cd31 primary antibodies ihc testing 3_1 pb9094 cd31 primary antibodies ihc testing 3_1](https://www.bosterbio.com/media/catalog/product/p/b/pb9094-cd31-primary-antibodies-ihc-testing-3_1.jpg)
Click image to see more details
Figure 3. IHC analysis of CD31/PECAM1 using anti-CD31/PECAM1 antibody (PB9094).
CD31/PECAM1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD31/PECAM1 Antibody (PB9094) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![pb9094 cd31 primary antibodies fcm testing 4 pb9094 cd31 primary antibodies fcm testing 4](https://www.bosterbio.com/media/catalog/product/p/b/pb9094-cd31-primary-antibodies-fcm-testing-4.jpg)
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Figure 4. Flow Cytometry analysis of Jurkat cells using anti-CD31/PECAM1 antibody (PB9094).
Overlay histogram showing Jurkat cells stained with PB9094 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD31/PECAM1 Antibody (PB9094, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PECAM1 (Source: Uniprot.org, NCBI)
Gene Name
PECAM1
Full Name
Platelet endothelial cell adhesion molecule
Weight
82536 MW
Alternative Names
adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule PECAM1 CD31, CD31/EndoCAM, GPIIA, PECA1, PECAM-1, endoCAM platelet and endothelial cell adhesion molecule 1 platelet endothelial cell adhesion molecule|CD31 antigen|platelet endothelial cell adhesion molecule-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PECAM1, check out the PECAM1 Infographic
![PECAM1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PECAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD31/PECAM1 Antibody Picoband® (PB9094)
Hello CJ!
PB9094 has been cited in 41 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Antibacterial polyvinyl alcohol/bacterial cellulose/nano-silver hydrogels that effectively promote wound healing
Tan PC,Chao PC,Cheng C,Chen CH,Huang RL,Zhou SB,Xie Y,Li QF.A randomized, controlled clinical trial of autologous stromal vascular fraction cells transplantation to promote mechanical stretch-induced skin regeneration.Stem Cell Res Ther.2021 Apr 15;12(1):243.doi:10.1186/s13287-021-02318-5.PMID:33858504;PMCID:P MC8048343.
Species: Human
PB9094 usage in article: APP:IHC, SAMPLE:SKIN TISSUE, DILUTION:1:100
Wang B,Lv X,Li Z,Zhang M,Yao J,Sheng N,Lu M,Wang H,Chen S.Urethra-inspired biomimetic scaffold: A therapeutic strategy to promote angiogenesis for urethral regeneration in a rabbit model.Acta Biomater.2020 Jan 15;102:247-258.doi:10.1016/j.actbio.2019.11.0
Species: Human,New Zealand Rabbit
PB9094 usage in article: APP:IHC, SAMPLE:HUVECS, DILUTION:1:500
EPCR promotes MGC803 human gastric cancer cell tumor angiogenesis in vitro through activating ERK1/2 and AKT in a PAR1%u2011dependent manner
MicroRNA-210 promotes angiogenesis in acute myocardial infarction
Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase
The induction of autophagy against mitochondria-mediated apoptosis in lung cancer cells by a ruthenium (II) imidazole complex
Wound Dressing Model of Human Umbilical Cord Mesenchymal Stem Cells-Alginates Complex Promotes Skin Wound Healing by Paracrine Signaling
ZBRK1, a novel tumor suppressor, activates VHL gene transcription through formation of a complex with VHL and p300 in renal cancer
Label-retaining assay enriches tumor-initiating cells in glioblastoma spheres cultivated in serum-free medium
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Customer Reviews
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1 Reviews For Anti-CD31/PECAM1 Antibody Picoband®
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Immunocytochemistry for Anti-CD31/PECAM1 Antibody
Excellent
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Application | Immunohistochemistry (paraffin-embedded) |
---|---|
Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
Sample | Human Bowel |
Fixative | Fixed with 4% paraformaldehyde |
Primary Ab Incubation | 37°C for 30 min |
Primary Ab Incubation diluent | 5% BSA in TBS |
Primary Ab Concentration | 1ug/ml |
Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
Secondary Ab Dilution | The kit was ready to use, no dilution needed |
Secondary Ab Incubation | at 37°C for 30 min |
J. Lewis
Verified customer
Submitted 2018-11-11
Customer Q&As
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16 Customer Q&As for Anti-CD31/PECAM1 Antibody Picoband®
Question
I was wanting to use your anti-CD31/PECAM1 antibody for IHC for human platelet on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human platelet identification?
L. Jones
Verified customer
Asked: 2020-04-08
Answer
As indicated on the product datasheet, PB9094 anti-CD31/PECAM1 antibody has been validated for Flow Cytometry, IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human platelet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-08
Question
Is there a BSA free version of anti-CD31/PECAM1 antibody PB9094 available?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD31/PECAM1 antibody PB9094 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-28
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-CD31/PECAM1 antibody PB9094. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-09
Question
My colleagues were happy with the WB result of your anti-CD31/PECAM1 antibody. However we have been able to see positive staining in lung cell membrane using this antibody. Is that expected? Could you tell me where is PECAM1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
According to literature, lung does express PECAM1. Generally PECAM1 expresses in cell membrane. Regarding which tissues have PECAM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2019-11-28
Question
We have seen staining in human liver. Do you have any suggestions? Is anti-CD31/PECAM1 antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
From literature liver does express PECAM1. From Uniprot.org, PECAM1 is expressed in tendon of biceps brachii, lung, brain, platelet, liver, leukemic t-cell, among other tissues. Regarding which tissues have PECAM1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973, 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 14702039
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2019-10-07
Question
I see that the anti-CD31/PECAM1 antibody PB9094 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-15
Question
Does anti-CD31/PECAM1 antibody PB9094 work for IHC with platelet?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
According to the expression profile of platelet, PECAM1 is highly expressed in platelet. So, it is likely that anti-CD31/PECAM1 antibody PB9094 will work for IHC with platelet.
Boster Scientific Support
Answered: 2019-06-14
Question
I have attached the WB image, lot number and protocol we used for platelet using anti-CD31/PECAM1 antibody PB9094. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-29
Question
My question regarding product PB9094, anti-CD31/PECAM1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-01
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9094 anti-CD31/PECAM1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-01
Question
Our lab want to know about to test anti-CD31/PECAM1 antibody PB9094 on human platelet for research purposes, then I may be interested in using anti-CD31/PECAM1 antibody PB9094 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Banerjee
Verified customer
Asked: 2018-03-13
Answer
The products we sell, including anti-CD31/PECAM1 antibody PB9094, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-13
Question
We ordered your anti-CD31/PECAM1 antibody for WB on leukemic t-cell in the past. I am using human, and We want to use the antibody for IHC next. you antibody examining leukemic t-cell as well as lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
B. Patel
Verified customer
Asked: 2017-10-25
Answer
I took a look at the website and datasheets of our anti-CD31/PECAM1 antibody and it appears that PB9094 has been validated on human in both WB and IHC. Thus PB9094 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-10-25
Question
We need using your anti-CD31/PECAM1 antibody for neutrophil degranulation studies. Has this antibody been tested with western blotting on jurkat whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-09-26
Answer
Thanks for your inquiry. This PB9094 anti-CD31/PECAM1 antibody is tested on hela whole cell lysate, u937 cells, mm231 whole cell lysate, jurkat whole cell lysate, raji whole cell lysate. It is guaranteed to work for Flow Cytometry, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-09-26
Question
We are currently using anti-CD31/PECAM1 antibody PB9094 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
V. Zhao
Verified customer
Asked: 2016-12-02
Answer
The anti-CD31/PECAM1 antibody (PB9094) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-12-02
Question
Will PB9094 anti-CD31/PECAM1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Patel
Verified customer
Asked: 2014-12-11
Answer
You can see on the product datasheet, PB9094 anti-CD31/PECAM1 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-12-11
Question
Is a blocking peptide available for product anti-CD31/PECAM1 antibody (PB9094)?
D. Williams
Verified customer
Asked: 2013-05-14
Answer
We do provide the blocking peptide for product anti-CD31/PECAM1 antibody (PB9094). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-05-14
Question
Is this PB9094 anti-CD31/PECAM1 antibody reactive to the isotypes of PECAM1?
H. Wu
Verified customer
Asked: 2013-01-03
Answer
The immunogen of PB9094 anti-CD31/PECAM1 antibody is E.coli-derived human CD31 recombinant protein (Position: Q28-G382). Human CD31 shares 65% and 68% amino acid (aa) sequences identity with mouse and rat CD31, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-01-03