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Product Info Summary
| SKU: | EK1625 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants,cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human GSTA ELISA Kit PicoKine®
SKU/Catalog Number
EK1625
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human GSTA ELISA Kit PicoKine® (96 Tests). Quantitate Human GSTA1 in cell culture supernatants,cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human GSTA ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1625)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A2-F222
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1625 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1625 is reactive to GSTA1 in Human samples
Validated Sample Types
cell culture supernatants,cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1625 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of GSTA1
GSTA encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1625-CAP | Anti-Human GSTA1 Pre-coated 96-well strip microplate | 1 |
| EK1625-ST | Human GSTA1 Standard | 2 vials, 10 ng/tube |
| EK1625-DA | Human GSTA1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 313 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.065 | 0.188 | 0.295 | 0.598 | 1.039 | 1.333 | 1.716 | 1.916 |
Data Example Images
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Human GSTA PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect GSTA1, Dilution ratio of 1:1, concentration in serum and plasma is 69-1000 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human GSTA ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 298 | 1176 | 6136 | 305 | 1197 | 6246 |
| Standard deviation | 13.1 | 55.27 | 331.3 | 19.22 | 80.2 | 462.2 |
| CV (%) | 4.4% | 4.7% | 5.4% | 6.3% | 6.7% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of GSTA1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 298 | 279 | 301 | 294 | 293 | 8.45 | 2.8% |
| Sample . 2 | 1176 | 1300 | 1194 | 1293 | 1240 | 56.16 | 4.5%% |
| Sample 3 | 6136 | 5932 | 6351 | 5515 | 5983 | 308.40 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For GSTA1 (Source: Uniprot.Org, NCBI)
Gene Name
GSTA1
Full Name
Glutathione S-transferase A1
Weight
25.631kDa
Superfamily
GST superfamily
Alternative Names
Glutathione S-transferase A2; GST HA subunit 2; GST class-alpha member 2; GST-gamma; GSTA2-2; GTH2; GSTA2; GST2 GSTA1 GST-epsilon, GST2-1, GTH1, GSTA1 glutathione S-transferase alpha 1 glutathione S-transferase A1|13-hydroperoxyoctadecadienoate peroxidase|GST HA subunit 1|GST class-alpha member 1|S-(hydroxyalkyl)glutathione lyase A1|androst-5-ene-3,17-dione isomerase|glutathione S-alkyltransferase A1|glutathione S-aryltransferase A1|glutathione S-transferase 2|glutathione S-transferase Ha subunit 1|testicular tissue protein Li 80
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GSTA1, check out the GSTA1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GSTA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human GSTA ELISA Kit PicoKine® (EK1625)
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Customer Q&As
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6 Customer Q&As for Human GSTA ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
S. Zhou
Verified customer
Asked: 2020-07-05
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-07-05
Question
Q: What is the optimal O.D. value for GSTA1 ELISA kit? I performed your GSTA1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain GSTA1 even though the O.D. values are not very high?
C. Zhao
Verified customer
Asked: 2020-06-02
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this GSTA1 ELISA kit to have sensitivity of 10pg/ml , that means the minimum amount of GSTA1 that can be declared/interpreted as positive by the above standard is 10pg/ml .
Boster Scientific Support
Answered: 2020-06-02
Question
Q: how to analyze ELISA data? I measured GSTA1 level in cell culture supernates,cell lysates.
Verified Customer
Verified customer
Asked: 2019-04-01
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-04-01
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of GSTA1 in Human cell culture supernates,cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
M. Krishna
Verified customer
Asked: 2018-07-11
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the GSTA1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-07-11
Question
Q: if the enzyme conjugated GSTA1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the GSTA1 antigen?
Verified Customer
Verified customer
Asked: 2018-02-02
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-02-02
Question
Q: how can I thaw whole blood sample for GSTA1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-02-21
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test GSTA1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-02-21
