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Product Info Summary
| SKU: | M01225 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC |
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Product info
Product Name
Anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal Antibody
View all CD30/TNFRSF8 Antibodies
SKU/Catalog Number
M01225
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal Antibody (Catalog # M01225). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01225)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM609
Isotype
IgG1, kappa
Immunogen
Recombinant human TNFRSF8 protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01225 is reactive to TNFRSF8 in Human
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
63747 MW
Background of CD30/TNFRSF8
Recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkin's disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This monoclonal antibody distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01225 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with Anti-CD30 Monoclonal Antibody (SPM609).
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF8 (Source: Uniprot.org, NCBI)
Gene Name
TNFRSF8
Full Name
Tumor necrosis factor receptor superfamily member 8
Weight
63747 MW
Superfamily
TNFR8 family
Alternative Names
Tumor necrosis factor receptor superfamily member 8;CD30L receptor;Ki-1 antigen;Lymphocyte activation antigen CD30;CD30;TNFRSF8;CD30, D1S166E; TNFRSF8 CD30, D1S166E, Ki-1 TNF receptor superfamily member 8 tumor necrosis factor receptor superfamily member 8|CD30L receptor|Ki-1 |cytokine receptor CD30|lymphocyte activation CD30
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFRSF8, check out the TNFRSF8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFRSF8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal Antibody (M01225)
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No publications found for M01225
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Customer Q&As
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15 Customer Q&As for Anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal Antibody
Question
Is there a BSA free version of anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 available?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-02
Question
See below the WB image, lot number and protocol we used for leukocyte using anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-24
Question
Is a blocking peptide available for product anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody (M01225)?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
We do provide the blocking peptide for product anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody (M01225). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-20
Question
Would M01225 anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
As indicated on the product datasheet, M01225 anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-10
Question
I was wanting to use your anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody for IHC for human leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukocyte identification?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
You can see on the product datasheet, M01225 anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody has been validated for IHC on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-05
Question
I see that the anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-24
Question
We were well pleased with the WB result of your anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody. However we have observed positive staining in leukemic t-cell isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is TNFRSF8 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
From literature, leukemic t-cell does express TNFRSF8. Generally TNFRSF8 expresses in isoform 1: cell membrane, isoform 2: cytoplasm. Regarding which tissues have TNFRSF8 expression, here are a few articles citing expression in various tissues:
Blood, and Testis, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoid tissue, Pubmed ID: 1310894
Placenta, Pubmed ID: 16710414
Boster Scientific Support
Answered: 2019-05-24
Question
We have observed staining in human placenta. What should we do? Is anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody supposed to stain placenta positively?
Verified Customer
Verified customer
Asked: 2019-03-28
Answer
Based on literature placenta does express TNFRSF8. Based on Uniprot.org, TNFRSF8 is expressed in leukocyte, lymphoid tissue, placenta, blood testis, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have TNFRSF8 expression, here are a few articles citing expression in various tissues:
Blood, and Testis, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoid tissue, Pubmed ID: 1310894
Placenta, Pubmed ID: 16710414
Boster Scientific Support
Answered: 2019-03-28
Question
Does anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 work for IHC with leukocyte?
G. Gonzalez
Verified customer
Asked: 2019-03-28
Answer
According to the expression profile of leukocyte, TNFRSF8 is highly expressed in leukocyte. So, it is likely that anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 will work for IHC with leukocyte.
Boster Scientific Support
Answered: 2019-03-28
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225. Let me know if you need anything else.
A. Singh
Verified customer
Asked: 2018-11-30
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-30
Question
Is this M01225 anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody reactive to the isotypes of TNFRSF8?
E. Taylor
Verified customer
Asked: 2018-11-19
Answer
The immunogen of M01225 anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody is Recombinant human TNFRSF8 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-11-19
Question
We are currently using anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-04
Answer
The anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody (M01225) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-04
Question
I have a question about product M01225, anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Carter
Verified customer
Asked: 2016-05-23
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01225 anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-05-23
Question
My lab would like to test anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 on human leukocyte for research purposes, then I may be interested in using anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Mitchell
Verified customer
Asked: 2013-10-29
Answer
The products we sell, including anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-10-29
Question
Will anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 work on primate IHC with blood testis?
C. Jones
Verified customer
Asked: 2013-08-01
Answer
Our lab technicians have not validated anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 on primate. You can run a BLAST between primate and the immunogen sequence of anti-CD30 / TNFRSF8 (Hodgkin & Reed-Sternberg Cell Marker) Monoclonal antibody M01225 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate blood testis in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-08-01
