Click image to see more details
Product Info Summary
| SKU: | RP1029 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD30/TNFRSF8 Antibody Picoband®
View all CD30/TNFRSF8 Antibodies
SKU/Catalog Number
RP1029
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD30/TNFRSF8 Antibody catalog # RP1029. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD30/TNFRSF8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1029)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD30 recombinant protein (Position: T322-K595). Human CD30 shares 60% and 57% amino acid (aa) sequences identity with mouse and rat CD30, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1029 is reactive to TNFRSF8 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
67 kDa
Calculated molecular weight
63747 MW
Background of CD30/TNFRSF8
CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1029 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA Whole Cell, 293T Whole Cell, JURKAT Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-CD30 Picoband antibody, RP1029.jpg
All lanes: Anti-CD30(RP1029) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 67KD
Observed bind size: 67KD
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF8 (Source: Uniprot.org, NCBI)
Gene Name
TNFRSF8
Full Name
Tumor necrosis factor receptor superfamily member 8
Weight
63747 MW
Superfamily
TNFR8 family
Alternative Names
Tumor necrosis factor receptor superfamily member 8;CD30L receptor;Ki-1 antigen;Lymphocyte activation antigen CD30;CD30;TNFRSF8;CD30, D1S166E; TNFRSF8 CD30, D1S166E, Ki-1 TNF receptor superfamily member 8 tumor necrosis factor receptor superfamily member 8|CD30L receptor|Ki-1 |cytokine receptor CD30|lymphocyte activation CD30
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFRSF8, check out the TNFRSF8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFRSF8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD30/TNFRSF8 Antibody Picoband® (RP1029)
Hello CJ!
No publications found for RP1029
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD30/TNFRSF8 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CD30/TNFRSF8 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Anti-CD30/TNFRSF8 Antibody Picoband®
Question
I see that the anti-CD30/TNFRSF8 antibody RP1029 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-03
Question
We are currently using anti-CD30/TNFRSF8 antibody RP1029 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
The anti-CD30/TNFRSF8 antibody (RP1029) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-13
Question
See attached the WB image, lot number and protocol we used for leukemic t-cell using anti-CD30/TNFRSF8 antibody RP1029. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-06
Question
Is this RP1029 anti-CD30/TNFRSF8 antibody reactive to the isotypes of TNFRSF8?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
The immunogen of RP1029 anti-CD30/TNFRSF8 antibody is E.coli-derived human CD30 recombinant protein (Position: T322-K595). Human CD30 shares 60% and 57% amino acid (aa) sequences identity with mouse and rat CD30, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-04
Question
Our lab were happy with the WB result of your anti-CD30/TNFRSF8 antibody. However we have seen positive staining in lymphoid tissue isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is TNFRSF8 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
From literature, lymphoid tissue does express TNFRSF8. Generally TNFRSF8 expresses in isoform 1: cell membrane, isoform 2: cytoplasm. Regarding which tissues have TNFRSF8 expression, here are a few articles citing expression in various tissues:
Blood, and Testis, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoid tissue, Pubmed ID: 1310894
Placenta, Pubmed ID: 16710414
Boster Scientific Support
Answered: 2019-07-16
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CD30/TNFRSF8 antibody RP1029. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-11
Question
I was wanting to use your anti-CD30/TNFRSF8 antibody for WB for human leukemic t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human leukemic t-cell identification?
B. Krishna
Verified customer
Asked: 2019-05-22
Answer
As indicated on the product datasheet, RP1029 anti-CD30/TNFRSF8 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-22
Question
Is a blocking peptide available for product anti-CD30/TNFRSF8 antibody (RP1029)?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
We do provide the blocking peptide for product anti-CD30/TNFRSF8 antibody (RP1029). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-08-02
Question
Does RP1029 anti-CD30/TNFRSF8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Gonzalez
Verified customer
Asked: 2018-07-12
Answer
It shows on the product datasheet, RP1029 anti-CD30/TNFRSF8 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-12
Question
We have seen staining in human lymphoid tissue. Any tips? Is anti-CD30/TNFRSF8 antibody supposed to stain lymphoid tissue positively?
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
From literature lymphoid tissue does express TNFRSF8. From Uniprot.org, TNFRSF8 is expressed in leukocyte, lymphoid tissue, placenta, blood testis, leukemic t-cell, erythroleukemia, among other tissues. Regarding which tissues have TNFRSF8 expression, here are a few articles citing expression in various tissues:
Blood, and Testis, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Lymphoid tissue, Pubmed ID: 1310894
Placenta, Pubmed ID: 16710414
Boster Scientific Support
Answered: 2018-05-18
Question
My question regarding product RP1029, anti-CD30/TNFRSF8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-05-15
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1029 anti-CD30/TNFRSF8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-15
Question
Do you have a BSA free version of anti-CD30/TNFRSF8 antibody RP1029 available?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD30/TNFRSF8 antibody RP1029 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-14
Question
We want using your anti-CD30/TNFRSF8 antibody for positive regulation of trail biosynthetic process studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-07-20
Answer
We appreciate your inquiry. This RP1029 anti-CD30/TNFRSF8 antibody is tested on hela whole cell lysate, 293t whole cell lysate, jurkat whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-07-20
Question
Does anti-CD30/TNFRSF8 antibody RP1029 work for WB with leukemic t-cell?
S. Krishna
Verified customer
Asked: 2017-03-29
Answer
According to the expression profile of leukemic t-cell, TNFRSF8 is highly expressed in leukemic t-cell. So, it is likely that anti-CD30/TNFRSF8 antibody RP1029 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2017-03-29
Question
My question regards to test anti-CD30/TNFRSF8 antibody RP1029 on human leukemic t-cell for research purposes, then I may be interested in using anti-CD30/TNFRSF8 antibody RP1029 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Carter
Verified customer
Asked: 2017-03-06
Answer
The products we sell, including anti-CD30/TNFRSF8 antibody RP1029, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-03-06
