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Product Info Summary
| SKU: | PB9931 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD27 Antibody Picoband®
View all CD27/TNFRSF7 Antibodies
SKU/Catalog Number
PB9931
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD27 Antibody Picoband® catalog # PB9931. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD27 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9931)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD27 recombinant protein (Position: A20-R191). Human CD27 shares 62.8% amino acid (aa) sequence identity with mouse CD27.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9931 is reactive to CD27 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
51 kDa
Calculated molecular weight
29137 MW
Background of CD27/TNFRSF7
CD27 is a member of the tumor necrosis factor receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. And this receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9931 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: HELA whole cell
IHC: human tonsil tissue
ICC/IF: K562 cell
FCM: A549 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD27 using anti-CD27 antibody (PB9931).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD27 antigen affinity purified polyclonal antibody (Catalog # PB9931) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD27 at approximately 51 kDa. The expected band size for CD27 is at 29 kDa.
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Figure 2. IHC analysis of CD27 using anti-CD27 antibody (PB9931).
CD27 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD27 Antibody (PB9931) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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3. Flow Cytometry analysis of A549 cells using anti-CD27 antibody (PB9931).
Overlay histogram showing A549 cells stained with PB9931 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD27 Antibody (PB9931,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Figure 4. IF analysis of CD27 using anti-CD27 antibody (PB9931).
CD27 was detected in immunocytochemical section of K562 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CD27 Antibody (PB9931) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CD27 (Source: Uniprot.org, NCBI)
Gene Name
CD27
Full Name
CD27 antigen
Weight
29137 MW
Alternative Names
CD27 antigen;CD27L receptor;T-cell activation antigen CD27;T14;Tumor necrosis factor receptor superfamily member 7;CD27;CD27;TNFRSF7; CD27 S152, S152. LPFS2, T14, TNFRSF7, Tp55 CD27 molecule CD27 |CD27L receptor|T cell activation S152|T-cell activation CD27|tumor necrosis factor receptor superfamily, member 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD27, check out the CD27 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD27 Antibody Picoband® (PB9931)
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No publications found for PB9931
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Customer Q&As
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16 Customer Q&As for Anti-CD27 Antibody Picoband®
Question
Can you help my question with product PB9931, anti-CD27 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9931 anti-CD27 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-05-08
Question
Will PB9931 anti-CD27 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Parker
Verified customer
Asked: 2020-05-05
Answer
As indicated on the product datasheet, PB9931 anti-CD27 antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-05
Question
Is a blocking peptide available for product anti-CD27 antibody (PB9931)?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
We do provide the blocking peptide for product anti-CD27 antibody (PB9931). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-13
Question
We have seen staining in mouse thymus. Any tips? Is anti-CD27 antibody supposed to stain thymus positively?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
According to literature thymus does express CD27. According to Uniprot.org, CD27 is expressed in lymph node, monocyte, thymus, b-cell, cervix carcinoma thymus, among other tissues. Regarding which tissues have CD27 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Cervix carcinoma, and Thymus, Pubmed ID: 9177220
Monocyte, Pubmed ID: 1655907
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-31
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma thymus using anti-CD27 antibody PB9931. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-26
Question
We ordered your anti-CD27 antibody for Flow Cytometry on monocyte in the past. I am using human, and We want to use the antibody for WB next. I am looking for examining monocyte as well as thymus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
I took a look at the website and datasheets of our anti-CD27 antibody and I see that PB9931 has been validated on human in both Flow Cytometry and WB. Thus PB9931 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-25
Question
Do you have a BSA free version of anti-CD27 antibody PB9931 available?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CD27 antibody PB9931 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-19
Question
Will anti-CD27 antibody PB9931 work for Flow Cytometry with cervix carcinoma thymus?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
According to the expression profile of cervix carcinoma thymus, CD27 is highly expressed in cervix carcinoma thymus. So, it is likely that anti-CD27 antibody PB9931 will work for Flow Cytometry with cervix carcinoma thymus.
Boster Scientific Support
Answered: 2019-09-24
Question
Our team were well pleased with the WB result of your anti-CD27 antibody. However we have seen positive staining in lymph node membrane using this antibody. Is that expected? Could you tell me where is CD27 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
From what I have seen in literature, lymph node does express CD27. Generally CD27 expresses in membrane. Regarding which tissues have CD27 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Cervix carcinoma, and Thymus, Pubmed ID: 9177220
Monocyte, Pubmed ID: 1655907
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-09-20
Question
I was wanting to use your anti-CD27 antibody for Flow Cytometry for human cervix carcinoma thymus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma thymus identification?
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
As indicated on the product datasheet, PB9931 anti-CD27 antibody has been tested for Flow Cytometry, IHC-P, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-29
Question
See attached the WB image, lot number and protocol we used for cervix carcinoma thymus using anti-CD27 antibody PB9931. Please let me know if you require anything else.
R. Collins
Verified customer
Asked: 2018-12-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-13
Question
We are currently using anti-CD27 antibody PB9931 for mouse tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
The anti-CD27 antibody (PB9931) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-04-05
Question
We are interested in to test anti-CD27 antibody PB9931 on human cervix carcinoma thymus for research purposes, then I may be interested in using anti-CD27 antibody PB9931 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-30
Answer
The products we sell, including anti-CD27 antibody PB9931, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-30
Question
I see that the anti-CD27 antibody PB9931 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-08-24
Question
Is this PB9931 anti-CD27 antibody reactive to the isotypes of CD27?
R. Anderson
Verified customer
Asked: 2014-10-28
Answer
The immunogen of PB9931 anti-CD27 antibody is E. coli-derived human CD27 recombinant protein (Position: A20-R191). Human CD27 shares 62.8% amino acid (aa) sequence identity with mouse CD27. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-10-28
Question
I am interested in using your anti-CD27 antibody for positive regulation of t cell differentiation studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.
W. Dhar
Verified customer
Asked: 2014-09-03
Answer
We appreciate your inquiry. This PB9931 anti-CD27 antibody is tested on hela whole cell lysates, a549 cells. It is guaranteed to work for Flow Cytometry, IHC-P, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-09-03
