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Product Info Summary
| SKU: | A01148-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC |
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Product info
Product Name
Anti-Cd27 Antibody
View all CD27/TNFRSF7 Antibodies
SKU/Catalog Number
A01148-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cd27 Antibody catalog # A01148-2. Tested in ELISA, Flow Cytometry, IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Cd27 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01148-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Cd27 recombinant protein (Position: P24-F187).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01148-2 is reactive to Cd27 in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
45214 MW
Background of CD27/TNFRSF7
CD27 is a member of the tumor necrosis factor receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. And this receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01148-2 is guaranteed for ELISA, Flow Cytometry, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/ml, -
Positive Control
IHC: mouse lymphaden tissue, rat lymphaden tissue, rat spleen tissue
FCM: mouse PBMC cell
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Cd27 using anti-Cd27 antibody (A01148-2).
Cd27 was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd27 Antibody (A01148-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 2. IHC analysis of Cd27 using anti-Cd27 antibody (A01148-2).
Cd27 was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd27 Antibody (A01148-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of Cd27 using anti-Cd27 antibody (A01148-2).
Cd27 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Cd27 Antibody (A01148-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 4. Flow Cytometry analysis of mouse PBMC cells using anti-Cd27 antibody (A01148-2).
Overlay histogram showing mouse PBMC cells stained with A01148-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Cd27 Antibody (A01148-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Cd27 (Source: Uniprot.org, NCBI)
Gene Name
Cd27
Full Name
CD27 antigen
Weight
45214 MW
Alternative Names
Angiopoietin-related protein 4;Angiopoietin-like protein 4;Hepatic fibrinogen/angiopoietin-related protein;HFARP;ANGPTL4;ARP4, HFARP, PGAR;PP1158, PSEC0166, UNQ171/PRO197; CD27 S152, S152. LPFS2, T14, TNFRSF7, Tp55 CD27 molecule CD27 |CD27L receptor|T cell activation S152|T-cell activation CD27|tumor necrosis factor receptor superfamily, member 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cd27, check out the Cd27 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cd27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cd27 Antibody (A01148-2)
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