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Product Info Summary
| SKU: | M03148 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-CD239 Rabbit Monoclonal Antibody
View all BCAM/CD239 Antibodies
SKU/Catalog Number
M03148
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-CD239 Rabbit Monoclonal Antibody catalog # M03148. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD239 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03148)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
22B46
Isotype
IgG
Immunogen
A synthesized peptide derived from human CD239
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03148 is reactive to BCAM in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
85 kDa
Calculated molecular weight
191615 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03148 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human HepG2 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of BCAM using anti-BCAM antibody (M03148).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCAM antigen affinity purified monoclonal antibody (M03148) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCAM at approximately 85 kDa. The expected band size for BCAM is at 67 kDa.
Protein Target Info & Infographic
Gene/Protein Information For BCAM (Source: Uniprot.org, NCBI)
Gene Name
BCAM
Full Name
Basal cell adhesion molecule
Weight
191615 MW
Alternative Names
Clathrin heavy chain 1;Clathrin heavy chain on chromosome 17;CLH-17;CLTC;CLH17, CLTCL2, KIAA0034; BCAM AU, CD239, LU, MSK19 basal cell adhesion molecule (Lutheran blood group) basal cell adhesion molecule|Auberger b |B-CAM cell surface glycoprotein|B-cell adhesion molecule|F8/G253 |Lutheran blood group variant LUGA|basal cell adhesion molecule (Lu and Au blood groups)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCAM, check out the BCAM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD239 Rabbit Monoclonal Antibody (M03148)
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