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Product Info Summary
| SKU: | M01767-5 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD133 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01767-5
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-CD133 Rabbit Monoclonal Antibody catalog # M01767-5. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD133 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01767-5)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
27P09
Isotype
IgG
Immunogen
A synthesized peptide derived from human CD133
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01767-5 is reactive to PROM1 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
115 kDa
Calculated molecular weight
97 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01767-5 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: human Caco-2 whole cell
IHC: human colon cancer tissue
Validation Images & Assay Conditions
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Figure 2. IHC analysis of CD133 using anti-CD133 antibody (M01767-5).
CD133 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-CD133 Antibody (M01767-5) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 1. Western blot analysis of CD133 using anti-CD133 antibody (M01767-5).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD133 antigen affinity purified monoclonal antibody (Catalog # M01767-5) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD133 at approximately 115 kDa. The expected band size for CD133 is at 97 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PROM1 (Source: Uniprot.org, NCBI)
Gene Name
PROM1
Full Name
Prominin-1
Weight
97 kDa
Superfamily
prominin family
Alternative Names
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial;1.3.5.1;Flavoprotein subunit of complex II;Fp;SDHA;SDH2, SDHF; PROM1 AC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4 prominin 1 prominin-1| AC133|hProminin|hematopoietic stem cell |prominin-like protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PROM1, check out the PROM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PROM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD133 Rabbit Monoclonal Antibody (M01767-5)
Hello CJ!
M01767-5 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Characterization of primary ovarian cancer cells in different culture systems
Stanniocalcin-1 augments stem-like traits of glioblastoma cells through binding and activating NOTCH1
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