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Product Info Summary
| SKU: | A04185-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-CCXCR1/XCR1 Antibody Picoband®
View all XCR1/CCXCR1 Antibodies
SKU/Catalog Number
A04185-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CCXCR1/XCR1 Antibody Picoband® catalog # A04185-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CCXCR1/XCR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04185-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CCXCR1/XCR1 recombinant protein (Position: F246-N274).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04185-1 is reactive to XCR1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
38.508kDa
Background of XCR1/CCXCR1
The protein encoded by this gene is a chemokine receptor belonging to the G protein-coupled receptor superfamily. The family members are characterized by the presence of 7 transmembrane domains and numerous conserved amino acids. This receptor is most closely related to RBS11 and the MIP1-alpha/RANTES receptor. It transduces a signal by increasing the intracellular calcium ions level. The viral macrophage inflammatory protein-II is an antagonist of this receptor and blocks signaling. Several alternatively spliced transcript variants encoding the same protein have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04185-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human RT4 whole cell, human U-87MG whole cell, human HEL whole cell, human U20S whole cell, rat spleen tissue, mouse spleen tissue, mouse thymus tissue
IHC: human spleen tissue, mouse thymus tissue, rat thymus tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CCXCR1/XCR1 using anti-CCXCR1/XCR1 antibody (A04185-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human U-87MG whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: mouse spleen tissue lysates,
Lane 7: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCXCR1/XCR1 antigen affinity purified polyclonal antibody (Catalog # A04185-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCXCR1/XCR1 at approximately 42 kDa. The expected band size for CCXCR1/XCR1 is at 39 kDa.
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Figure 2. IHC analysis of CCXCR1/XCR1 using anti-CCXCR1/XCR1 antibody (A04185-1).
CCXCR1/XCR1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCXCR1/XCR1 Antibody (A04185-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of CCXCR1/XCR1 using anti-CCXCR1/XCR1 antibody (A04185-1).
CCXCR1/XCR1 was detected in a paraffin-embedded section of mouse thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCXCR1/XCR1 Antibody (A04185-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of CCXCR1/XCR1 using anti-CCXCR1/XCR1 antibody (A04185-1).
CCXCR1/XCR1 was detected in a paraffin-embedded section of rat thymus tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CCXCR1/XCR1 Antibody (A04185-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For XCR1 (Source: Uniprot.org, NCBI)
Gene Name
XCR1
Full Name
Chemokine XC receptor 1
Weight
38.508kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
NADH-ubiquinone oxidoreductase chain 4; NADH dehydrogenase subunit 4; Mtnd4; mt-Nd4; Nd4 XCR1 CCXCR1, GPR5 X-C motif chemokine receptor 1 chemokine XC receptor 1|G protein-coupled receptor 5|XC chemokine receptor 1|chemokine (C motif) XC receptor 1|chemokine (C motif) receptor 1|lymphotactin receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on XCR1, check out the XCR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for XCR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CCXCR1/XCR1 Antibody Picoband® (A04185-1)
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