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Product Info Summary
| SKU: | A00158-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CCR2 Antibody Picoband®
SKU/Catalog Number
A00158-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CCR2 Antibody Picoband® catalog # A00158-4. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CCR2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00158-4)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CCR2 recombinant protein (Position: M1-F125).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00158-4 is reactive to CCR2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
42.783kDa
Background of Ccr2
C-C chemokine receptor type 2 (CCR2 or CD192 (cluster of differentiation 192) is a protein that in humans is encoded by the CCR2 gene. It is mapped to 3p21.31. The protein encoded by this gene is a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The encoded protein mediates agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This protein can also be a coreceptor with CD4 for HIV-1 infection.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00158-4 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human U-87MG whole cell, human K562 whole cell, human A549 whole cell, rat spleen issue, rat kidney issue, mouse spleen issue
Validation Images & Assay Conditions
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Boster Kit Box
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Figure 1. Western blot analysis of CCR2 using anti-CCR2 antibody (A00158-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat spleen issue lysates,
Lane 5: rat kidney issue lysates,
Lane 6: mouse spleen issue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR2 antigen affinity purified polyclonal antibody (Catalog # A00158-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR2 at approximately 45KD. The expected band size for CCR2 is at 42KD.
Protein Target Info & Infographic
Gene/Protein Information For CCR2 (Source: Uniprot.org, NCBI)
Gene Name
CCR2
Full Name
C-C chemokine receptor type 2
Weight
42.783kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CCR-2; CCR2; Monocyte chemoattractant protein 1 receptor; MCP-1-R; CD192; CCR2; CMKBR2 Ccr2|CC-CKR-, CCR, CKR, Cc-ckr-2a, Ccr2b, Ckr2, Ckr2a, Ckr2b, Cmkbr, Cmkbr2, mJe-r, Ccr2|chemokine (C-C motif) receptor 2|C-C chemokine receptor type 2|C-C CHEMOKINE RECEPTOR TYPE 2 (C-C CKR-2) (CC-CKR-2) (CCR-2) (CCR2) (JE/FIC RECEPTOR) (MCP-1 RECEPTOR)|C-C CKR-2|CCR-2|JE/FIC receptor|MCP-1 receptor|MIP-1 alphaR|chemoattractant protein-1 receptor|chemokine (C-C) receptor 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCR2, check out the CCR2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCR2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CCR2 Antibody Picoband® (A00158-4)
Hello CJ!
A00158-4 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
N%u2011methyl%u2011N%u2011nitro%u2011N'%u2011nitrosoguanidine induces the expression of CCR2 in human gastric epithelial cells promoting CCL2%u2011mediated migration
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CCR2 Antibody Picoband®
Question
Will anti-CCR2 antibody A00158-4 work for ELISA with leukocyte?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
According to the expression profile of leukocyte, CCR2 is highly expressed in leukocyte. So, it is likely that anti-CCR2 antibody A00158-4 will work for ELISA with leukocyte.
Boster Scientific Support
Answered: 2019-10-23
Question
Would A00158-4 anti-CCR2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
As indicated on the product datasheet, A00158-4 anti-CCR2 antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-09
Question
Is this A00158-4 anti-CCR2 antibody reactive to the isotypes of CCR2?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
The immunogen of A00158-4 anti-CCR2 antibody is E.coli-derived human CCR2 recombinant protein (Position: M1-F125). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-02
Question
See below the WB image, lot number and protocol we used for leukocyte using anti-CCR2 antibody A00158-4. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-20
Question
Is a blocking peptide available for product anti-CCR2 antibody (A00158-4)?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
We do provide the blocking peptide for product anti-CCR2 antibody (A00158-4). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-19
Question
I was wanting to use your anti-CCR2 antibody for ELISA for mouse leukocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse leukocyte identification?
Verified Customer
Verified customer
Asked: 2018-11-08
Answer
As indicated on the product datasheet, A00158-4 anti-CCR2 antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-08
Question
We have seen staining in rat thymus trachea. Any tips? Is anti-CCR2 antibody supposed to stain thymus trachea positively?
Verified Customer
Verified customer
Asked: 2018-11-07
Answer
From literature thymus trachea does express CCR2. From Uniprot.org, CCR2 is expressed in leukocyte, thymus trachea, among other tissues. Regarding which tissues have CCR2 expression, here are a few articles citing expression in various tissues:
Thymus, and Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-11-07
Question
I am looking for using your anti-CCR2 antibody for positive regulation of cold-induced thermogenesis studies. Has this antibody been tested with western blotting on k562 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-06-07
Answer
Thanks for your inquiry. This A00158-4 anti-CCR2 antibody is validated on human a549, k562 whole cell lysates, a549 whole cell lysates, rat kidney. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-06-07
Question
We ordered your anti-CCR2 antibody for WB on leukocyte a few years ago. I am using human, and We are going to use the antibody for ELISA next. I was wanting to use examining leukocyte as well as thymus trachea in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
I looked at the website and datasheets of our anti-CCR2 antibody and it appears that A00158-4 has been validated on human in both WB and ELISA. Thus A00158-4 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-04-30
Question
I see that the anti-CCR2 antibody A00158-4 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-08
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-08
Question
Do you have a BSA free version of anti-CCR2 antibody A00158-4 available?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CCR2 antibody A00158-4 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-01
Question
I have a question about product A00158-4, anti-CCR2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Miller
Verified customer
Asked: 2017-12-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00158-4 anti-CCR2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-20
Question
My colleagues were happy with the WB result of your anti-CCR2 antibody. However we have been able to see positive staining in leukocyte cell membrane using this antibody. Is that expected? Could you tell me where is CCR2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-09-12
Answer
From what I have seen in literature, leukocyte does express CCR2. Generally CCR2 expresses in cell membrane. Regarding which tissues have CCR2 expression, here are a few articles citing expression in various tissues:
Thymus, and Trachea, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-09-12
Question
We are currently using anti-CCR2 antibody A00158-4 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-21
Answer
The anti-CCR2 antibody (A00158-4) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-21
Question
you antibody to test anti-CCR2 antibody A00158-4 on mouse leukocyte for research purposes, then I may be interested in using anti-CCR2 antibody A00158-4 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Krishna
Verified customer
Asked: 2015-04-08
Answer
The products we sell, including anti-CCR2 antibody A00158-4, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-04-08
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-CCR2 antibody A00158-4. Let me know if you need anything else.
G. Johnson
Verified customer
Asked: 2013-04-19
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-04-19
