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Product Info Summary
| SKU: | A33349 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC |
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Product info
Product Name
Anti-Ccl21a Antibody
View all CCL21/6Ckine Antibodies
SKU/Catalog Number
A33349
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Ccl21a Antibody catalog # A33349. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Ccl21a Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A33349)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Ccl21a recombinant protein (Position: S24-G133).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A33349 is reactive to Ccl21a in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
14 kDa
Calculated molecular weight
14377 MW
Background of CCL21/6Ckine
This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A33349 is guaranteed for ELISA, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
IHC: mouse lung tissue, rat lung tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of Ccl21a using anti-Ccl21a antibody (A33349).
Ccl21a was detected in a paraffin-embedded section of mouse lung tissue tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ccl21a Antibody (A33349) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 2. IHC analysis of Ccl21a using anti-Ccl21a antibody (A33349).
Ccl21a was detected in a paraffin-embedded section of rat lung tissue tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Ccl21a Antibody (A33349) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Ccl21a (Source: Uniprot.org, NCBI)
Gene Name
Ccl21a
Full Name
C-C motif chemokine 21a
Weight
14377 MW
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
NADH-ubiquinone oxidoreductase chain 2; NADH dehydrogenase subunit 2; Mtnd2; mt-Nd2; Nd2 Ccl21a|6CKBAC, 6CKBAC2, 6Cki, 6Ckine, ALP, AW987545, CKb9, Exodus, Gm1987, S, SCYA21a, SLC, Scya, Scya2, Scya21, Scya21b, Tca, Tca4, plt|chemokine (C-C motif) ligand 21A (serine)|C-C motif chemokine 21a|CC chemokine 6Ckine-ser|CCL21-Ser|beta-chemokine exodus-2|exodus-2|small inducible cytokine A21a|thymus-derived chemotactic agent 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ccl21a, check out the Ccl21a Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ccl21a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Ccl21a Antibody (A33349)
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