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Product Info Summary
| SKU: | A02957-2 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-CCK4 (PTK7) Antibody (N-term)
SKU/Catalog Number
A02957-2
Size
80 µl
Description
Boster Bio Anti-CCK4 (PTK7) Antibody (N-term) (Catalog # A02957-2). Tested in IHC-P, Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CCK4 (PTK7) Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02957-2)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CCK4 (PTK7) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 21-52 amino acids from the N-terminal region of human CCK4 (PTK7).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02957-2 is reactive to PTK7 in Human
Applications
A02957-2 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
118392 Da
Background of PTK7/CCK4
CCK4 may function as a cell adhesion molecule. Although it belongs to the insuline receptor subfamily of the Tyr protein kinases, it likely lacks the catalytic activity of a tyrosine kinase. It may be connected to the pathophysiology of colon carcinomas and/or may represent a tumor progression marker. This Type I membrane protein is highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes, but weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle, and not in colon. It is also expressed in erythroleukemia cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000-1:2000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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All lanes : Anti-CCK4 (PTK7) Antibody (N-term) at 1:1000-1:2000 dilution
Lane 1: A431 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: Caco2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 118 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Flow cytometric analysis of NCI-H460 cells using CCK4 (PTK7) Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For PTK7 (Source: Uniprot.org, NCBI)
Gene Name
PTK7
Full Name
Inactive tyrosine-protein kinase 7
Weight
118392 Da
Superfamily
protein kinase superfamily
Alternative Names
CCK4; CCK-4; CCK4Colon carcinoma kinase 4; colon carcinoma kinase-4; Protein-tyrosine kinase 7; Pseudo tyrosine kinase receptor 7; PTK7 protein tyrosine kinase 7; PTK7; Tyrosine-protein kinase-like 7 PTK7 CCK-4, CCK4 protein tyrosine kinase 7 (inactive) inactive tyrosine-protein kinase 7|PTK7 protein tyrosine kinase 7|colon carcinoma kinase 4|pseudo tyrosine kinase receptor 7|tyrosine-protein kinase-like 7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTK7, check out the PTK7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTK7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CCK4 (PTK7) Antibody (N-term) (A02957-2)
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