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Product Info Summary
| SKU: | EK2058 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse SEMA6C ELISA Kit PicoKine®
View all Semaphorin 6C ELISA kits
SKU/Catalog Number
EK2058
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse SEMA6C ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Sema6c in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse SEMA6C ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2058)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A26-P605
Sensitivity
<50 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2058 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2058 is reactive to Sema6c in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2058 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Semaphorin 6C
Semaphorin-6C is a protein that in humans is encoded by the SEMA6C gene. This gene is mapped to 3; 3 F2.1. This gene product is a member of the semaphoring family of proteins. Semaphorins represent important molecular signals controlling multiple aspects of the cellular response that follows CNS injury, and thus may play an important role in neural regeneration.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2058-CAP | Anti-Mouse Sema6c Pre-coated 96-well strip microplate | 1 |
| EK2058-ST | Mouse Sema6c Standard | 2 vials, 10 ng/tube |
| EK2058-DA | Mouse Sema6c Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.056 | 0.123 | 0.164 | 0.286 | 0.527 | 0.928 | 1.613 | 2.354 |
Data Example Images
Click image to see more details
Mouse SEMA6C PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse SEMA6C ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 95 | 439 | 1073 | 104 | 465 | 1124 |
| Standard deviation | 4.56 | 18.88 | 43.99 | 6.86 | 26.97 | 85.42 |
| CV (%) | 4.8% | 4.3% | 4.1% | 6.6% | 5.8% | 7.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Semaphorin 6C in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 95 | 85 | 100 | 85 | 91 | 6.49 | 7.1% |
| Sample 2 | 439 | 446 | 458 | 491 | 458 | 19.95 | 4.3% |
| Sample 3 | 1073 | 1102 | 1194 | 1246 | 1153 | 69.51 | 6.0% |
Protein Target Info & Infographic
Gene/Protein Information For Sema6c (Source: Uniprot.Org, NCBI)
Gene Name
Sema6c
Full Name
Semaphorin-6C
Weight
99.686kDa
Superfamily
semaphorin family
Alternative Names
Semaphorin-6C; Semaphorin-Y; Sema Y SEMA6C SEMAY, m-SemaY, m-SemaY2 semaphorin 6C semaphorin-6C|sema Y|sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C|semaphorin-Y
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Sema6c, check out the Sema6c Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Sema6c: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse SEMA6C ELISA Kit PicoKine® (EK2058)
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Mouse SEMA6C ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for Semaphorin 6C ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-05-31
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Semaphorin 6C for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-05-31
Question
Q: Can Semaphorin 6C ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-11-29
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
B. Turner
Verified customer
Asked: 2019-09-02
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-09-02
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-08-25
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-08-25
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Semaphorin 6C using the EK2058 Mouse Semaphorin 6C Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-09-06
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2018-09-06
Question
Q: how to analyze ELISA data? I have obtained Semaphorin 6C level in serum.
Verified Customer
Verified customer
Asked: 2018-08-28
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-08-28
Question
Q: What is the optimal O.D. value for Semaphorin 6C ELISA kit? I used your Semaphorin 6C ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Semaphorin 6C even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-08-25
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Semaphorin 6C ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Semaphorin 6C that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2018-08-25
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
S. King
Verified customer
Asked: 2018-07-08
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-07-08
Question
Q: is it okay to use citrate plasma as samples in Mouse Semaphorin 6C Picokine® ELISA Kit (Catalog # EK2058)?
Verified Customer
Verified customer
Asked: 2017-06-17
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Semaphorin 6C causing degradation of its protein structure. Semaphorin 6C may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Semaphorin 6C ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-06-17
