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Product Info Summary
| SKU: | A14619 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-CCDC134 Antibody
SKU/Catalog Number
A14619
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CCDC134 Antibody (Catalog # A14619). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CCDC134 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CCDC134 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14619)
Host
Rabbit
Contents
CCDC134 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CCDC134 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CCDC134. The immunogen is located within the first 50 amino acids of CCDC134.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of ZBTB5 are known to exist; this antibody will recognize both isoforms. This antibody is predicted to not cross-react with other ZBTB protein family members.
Reactive Species
A14619 is reactive to CCDC134 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
26561 MW
Background of Ccdc134
The coiled-coil domain is a common protein motif that is often involved in protein oligomerization and is found in proteins such as transcription factors and intermediate filaments. One such protein is CCDC134, a recently identified secretory protein that has been found to inhibit the transcriptional activity of the Elk1 protein. Overexpression CCDC134 also inhibited the phosphorylation of Erk and JNK/SAPK but not p38 MAPK, while specific siRNA against CCDC134 activated Elk1 transcriptional activity and the phosphorylation of Erk and JNK/SAPK, suggesting a potential inhibiting role of CCDC134 in MAPK-mediated Elk1 transcription. CCDC134 is widely expressing in normal adult tissues, tumors, and cell lines.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14619 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CCDC134 antibody can be used for detection of CCDC134 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CCDC134 in rat brain tissue lysate with CCDC134 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of CCDC134 in human brain tissue with CCDC134 antibody at 2.5 μg/mL.
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Immunofluorescence of CDCC134 in human brain tissue with CDCC134 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CCDC134 (Source: Uniprot.org, NCBI)
Gene Name
CCDC134
Full Name
Coiled-coil domain-containing protein 134
Weight
26561 MW
Superfamily
CCDC134 family
Alternative Names
dJ821D11.3, Coiled-coil domain-containing protein 134
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCDC134, check out the CCDC134 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCDC134: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CCDC134 Antibody (A14619)
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