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Product Info Summary
| SKU: | M00179 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Caveolin-1 CAV1 Rabbit Monoclonal Antibody
View all Caveolin-1 Antibodies
SKU/Catalog Number
M00179
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Caveolin-1 CAV1 Rabbit Monoclonal Antibody catalog # M00179. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caveolin-1 CAV1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00179)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Caveolin-1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00179 is reactive to CAV1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa
Calculated molecular weight
20472 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00179 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:20
Positive Control
IHC: mouse lung tissue
ICC/IF: A431 cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
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Immunofluorescent analysis of A431 cells, using Caveolin-1 Antibody .
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Western blot analysis of Caveolin-1 expression in A431 cell lysate.
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded mouse lung, using Caveolin-1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For CAV1 (Source: Uniprot.org, NCBI)
Gene Name
CAV1
Full Name
Caveolin-1
Weight
20472 MW
Superfamily
caveolin family
Alternative Names
Caveolin-1;CAV1;CAV; CAV1 BSCL3, CGL3, LCCNS, MSTP085, PPH3, VIP21 caveolin 1 caveolin-1|caveolin 1, caveolae protein, 22kDa|cell growth-inhibiting protein 32
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAV1, check out the CAV1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAV1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caveolin-1 CAV1 Rabbit Monoclonal Antibody (M00179)
Hello CJ!
M00179 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
MicroRNA-199a-5p Affects Porcine Preadipocyte Proliferation and Differentiation
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Caveolin-1 CAV1 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-Caveolin-1 Rabbit Monoclonal antibody (M00179)?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
We do provide the blocking peptide for product anti-Caveolin-1 Rabbit Monoclonal antibody (M00179). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-07
Question
Is this M00179 anti-Caveolin-1 Rabbit Monoclonal antibody reactive to the isotypes of CAV1?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
The immunogen of M00179 anti-Caveolin-1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Caveolin-1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-24
Question
We are currently using anti-Caveolin-1 Rabbit Monoclonal antibody M00179 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
The anti-Caveolin-1 Rabbit Monoclonal antibody (M00179) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-17
Question
We were happy with the WB result of your anti-Caveolin-1 Rabbit Monoclonal antibody. However we have seen positive staining in lung golgi apparatus membrane using this antibody. Is that expected? Could you tell me where is CAV1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
According to literature, lung does express CAV1. Generally CAV1 expresses in golgi apparatus membrane. Regarding which tissues have CAV1 expression, here are a few articles citing expression in various tissues:
Adipocyte, Pubmed ID: 15242332
Adipose tissue, Pubmed ID: 15219854
Brain, Pubmed ID: 11865038, 12531427
Brain, and Lung, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 1360410
Boster Scientific Support
Answered: 2020-02-28
Question
I was wanting to use your anti-Caveolin-1 Rabbit Monoclonal antibody for IF for human visceral pleura on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human visceral pleura identification?
T. Parker
Verified customer
Asked: 2020-01-02
Answer
You can see on the product datasheet, M00179 anti-Caveolin-1 Rabbit Monoclonal antibody has been tested for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human visceral pleura in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-02
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for visceral pleura using anti-Caveolin-1 Rabbit Monoclonal antibody M00179. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-30
Question
Does M00179 anti-Caveolin-1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
You can see on the product datasheet, M00179 anti-Caveolin-1 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-20
Question
We are interested in using your anti-Caveolin-1 Rabbit Monoclonal antibody for positive regulation of nf-kappab transcription factor activity studies. Has this antibody been tested with western blotting on mouse lung? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
I appreciate your inquiry. This M00179 anti-Caveolin-1 Rabbit Monoclonal antibody is tested on mouse lung, a431 cell lysate. It is guaranteed to work for IF, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-06-26
Question
I see that the anti-Caveolin-1 Rabbit Monoclonal antibody M00179 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-10
Question
Here is the WB image, lot number and protocol we used for visceral pleura using anti-Caveolin-1 Rabbit Monoclonal antibody M00179. Please let me know if you require anything else.
A. Huang
Verified customer
Asked: 2018-11-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-22
Question
My question regarding product M00179, anti-Caveolin-1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-02-08
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00179 anti-Caveolin-1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-02-08
Question
you antibody to test anti-Caveolin-1 Rabbit Monoclonal antibody M00179 on human visceral pleura for research purposes, then I may be interested in using anti-Caveolin-1 Rabbit Monoclonal antibody M00179 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-12
Answer
The products we sell, including anti-Caveolin-1 Rabbit Monoclonal antibody M00179, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-12
Question
Will anti-Caveolin-1 Rabbit Monoclonal antibody M00179 work for IF with visceral pleura?
Verified Customer
Verified customer
Asked: 2017-06-05
Answer
According to the expression profile of visceral pleura, CAV1 is highly expressed in visceral pleura. So, it is likely that anti-Caveolin-1 Rabbit Monoclonal antibody M00179 will work for IF with visceral pleura.
Boster Scientific Support
Answered: 2017-06-05
Question
Our lab used your anti-Caveolin-1 Rabbit Monoclonal antibody for IHC on brain a few months ago. I am using human, and We want to use the antibody for IF next. I would like examining brain as well as visceral pleura in our next experiment. Could give a recommendation on which antibody would work the best for IF?
M. Yang
Verified customer
Asked: 2016-01-18
Answer
I have checked the website and datasheets of our anti-Caveolin-1 Rabbit Monoclonal antibody and it appears that M00179 has been validated on human in both IHC and IF. Thus M00179 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-01-18
Question
Do you have a BSA free version of anti-Caveolin-1 Rabbit Monoclonal antibody M00179 available?
L. Zhang
Verified customer
Asked: 2015-12-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Caveolin-1 Rabbit Monoclonal antibody M00179 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-12-29
Question
We have seen staining in human brain. Any tips? Is anti-Caveolin-1 Rabbit Monoclonal antibody supposed to stain brain positively?
M. Kulkarni
Verified customer
Asked: 2013-08-13
Answer
From literature brain does express CAV1. From Uniprot.org, CAV1 is expressed in visceral pleura, lung, brain lung, adipose tissue, adipocyte, brain, cervix carcinoma, liver, among other tissues. Regarding which tissues have CAV1 expression, here are a few articles citing expression in various tissues:
Adipocyte, Pubmed ID: 15242332
Adipose tissue, Pubmed ID: 15219854
Brain, Pubmed ID: 11865038, 12531427
Brain, and Lung, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 1360410
Boster Scientific Support
Answered: 2013-08-13
