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Product Info Summary
| SKU: | M00179-2 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Caveolin-1 CAV1 Rabbit Monoclonal Antibody, Clone#RM325
View all Caveolin-1 Antibodies
SKU/Catalog Number
M00179-2
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Caveolin-1 CAV1 Rabbit Monoclonal Antibody, Clone#RM325 (Catalog # M00179-2). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caveolin-1 CAV1 Rabbit Monoclonal Antibody, Clone#RM325 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00179-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM325
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human Caveolin-1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human Caveolin-1.
Reactive Species
M00179-2 is reactive to CAV1 in Human
Reconstitution
Calculated molecular weight
20472 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00179-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:5,000-1:40,000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of HeLa cell lysate using anti-Caveolin-1 rabbit monoclonal antibody (Clone RM325) at a 1:40,000 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human liver tissue section using anti-Caveolin-1 rabbit monoclonal antibody (Clone RM325) at a 1:1000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CAV1 (Source: Uniprot.org, NCBI)
Gene Name
CAV1
Full Name
Caveolin-1
Weight
20472 MW
Superfamily
caveolin family
Alternative Names
Caveolin-1;CAV1;CAV; CAV1 BSCL3, CGL3, LCCNS, MSTP085, PPH3, VIP21 caveolin 1 caveolin-1|caveolin 1, caveolae protein, 22kDa|cell growth-inhibiting protein 32
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAV1, check out the CAV1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAV1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caveolin-1 CAV1 Rabbit Monoclonal Antibody, Clone#RM325 (M00179-2)
Hello CJ!
M00179-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
MicroRNA-199a-5p Affects Porcine Preadipocyte Proliferation and Differentiation
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Caveolin-1 CAV1 Rabbit Monoclonal Antibody, Clone#RM325
Question
Is a blocking peptide available for product anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 (M00179-2)?
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
We do provide the blocking peptide for product anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 (M00179-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-06
Question
Do you have a BSA free version of anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 M00179-2 available?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 M00179-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-10
Question
Does M00179-2 anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
You can see on the product datasheet, M00179-2 anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-10
Question
We have been able to see staining in human cervix carcinoma. Do you have any suggestions? Is anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
Based on literature cervix carcinoma does express CAV1. Based on Uniprot.org, CAV1 is expressed in visceral pleura, lung, brain lung, adipose tissue, adipocyte, brain, cervix carcinoma, liver, among other tissues. Regarding which tissues have CAV1 expression, here are a few articles citing expression in various tissues:
Adipocyte, Pubmed ID: 15242332
Adipose tissue, Pubmed ID: 15219854
Brain, Pubmed ID: 11865038, 12531427
Brain, and Lung, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 1360410
Boster Scientific Support
Answered: 2019-10-28
Question
Would anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 M00179-2 work for IHC with visceral pleura?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
According to the expression profile of visceral pleura, CAV1 is highly expressed in visceral pleura. So, it is likely that anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 M00179-2 will work for IHC with visceral pleura.
Boster Scientific Support
Answered: 2019-10-23
Question
I have a question about product M00179-2, anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00179-2 anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-10
Question
Is this M00179-2 anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 reactive to the isotypes of CAV1?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
The immunogen of M00179-2 anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 is A peptide corresponding to the C-terminus of human Caveolin-1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-13
Question
We bought anti-Caveolin-1 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
I viewed the website and datasheets of our anti-Caveolin-1 Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-08-21
Question
We want using your anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 for negative regulation of receptor signaling pathway via jak-stat studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
W. Singh
Verified customer
Asked: 2019-07-04
Answer
Thanks for your inquiry. This M00179-2 anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 is tested on hela cell lysate, human liver tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-07-04
Question
I was wanting to use your anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 for IHC for human visceral pleura on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human visceral pleura identification?
J. Krishna
Verified customer
Asked: 2019-03-18
Answer
You can see on the product datasheet, M00179-2 anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human visceral pleura in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-18
Question
We want to test anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 M00179-2 on human visceral pleura for research purposes, then I may be interested in using anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 M00179-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-28
Answer
The products we sell, including anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 M00179-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-28
Question
My boss were content with the WB result of your anti-Caveolin-1 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2018-09-26
Answer
Based on literature, cervix carcinoma does express CAV1. Generally CAV1 expresses in golgi apparatus membrane. Regarding which tissues have CAV1 expression, here are a few articles citing expression in various tissues:
Adipocyte, Pubmed ID: 15242332
Adipose tissue, Pubmed ID: 15219854
Brain, Pubmed ID: 11865038, 12531427
Brain, and Lung, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231, 23186163
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 1360410
Boster Scientific Support
Answered: 2018-09-26
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for visceral pleura using anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 M00179-2. Let me know if you need anything else.
K. Miller
Verified customer
Asked: 2018-08-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-14
Question
I see that the anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 M00179-2 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-05-30
Question
See below the WB image, lot number and protocol we used for visceral pleura using anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 M00179-2. Please let me know if you require anything else.
H. Miller
Verified customer
Asked: 2017-07-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-18
Question
We are currently using anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 M00179-2 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
R. Miller
Verified customer
Asked: 2013-11-11
Answer
The anti-Caveolin-1 Rabbit Monoclonal antibody, Clone#RM325 (M00179-2) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-11-11
