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Product Info Summary
| SKU: | PB9924 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Calcium Sensing Receptor/CASR Antibody Picoband®
View all Calcium-sensing R/CaSR Antibodies
SKU/Catalog Number
PB9924
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Calcium Sensing Receptor/CASR Antibody Picoband® catalog # PB9924. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calcium Sensing Receptor/CASR Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9924)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CASR recombinant protein (Position: Q926-S1078). Human CASR shares 80.5% and 78.6% amino acid (aa) sequence identity with mouse and rat CASR, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9924 is reactive to CASR in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated molecular weight
120674 MW
Background of Calcium-sensing R/CaSR
The calcium-sensing receptor (CaSR) is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9924 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: HELA whole cell, 22RV1 whole cell
IHC: mouse kidney tissue, rat kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CASR using anti-CASR antibody (PB9924).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: 22RV1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASR antigen affinity purified polyclonal antibody (Catalog # PB9924) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASR at approximately 150 kDa. The expected band size for CASR is at 121 kDa.
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Figure 2. IHC analysis of CASR using anti-CASR antibody (PB9924).
CASR was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CASR Antibody (PB9924) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of CASR using anti-CASR antibody (PB9924).
CASR was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CASR Antibody (PB9924) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CASR (Source: Uniprot.org, NCBI)
Gene Name
CASR
Full Name
Extracellular calcium-sensing receptor
Weight
120674 MW
Superfamily
G-protein coupled receptor 3 family
Alternative Names
Extracellular calcium-sensing receptor;CaSR;Parathyroid cell calcium-sensing receptor 1;PCaR1;CASR;GPRC2A, PCAR1; CASR CAR, EIG8, FHH, FIH, GPRC2A, HHC, HHC1, HYPOC1, NSHPT, PCAR1, hCasR calcium sensing receptor extracellular calcium-sensing receptor|parathyroid Ca(2+)-sensing receptor 1|parathyroid cell calcium-sensing receptor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASR, check out the CASR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calcium Sensing Receptor/CASR Antibody Picoband® (PB9924)
Hello CJ!
PB9924 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wouter H. van Megen,Rebecca Tan,R. Todd Alexander,Henrik Dimke bioRxiv 2021.02.01.429170; doi: https://doi.org/10.1101/2021.02.01.429170
Species: Human,Mouse
PB9924 usage in article: APP:IHC, SAMPLE:PARATHYROID AND RENAL CORTEX, DILUTION:NA
Yuan, H., Shi, Y., Li, Y., Yang, L., Tang, Y., Hu, Z.,…, & Tang, C. (2017). Evaluation of a TPTX model induced by ischemia. Experimental Animals. Advance online publication. doi: 10.1538/expanim.17-0029
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Customer Q&As
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1 Customer Q&As for Anti-Calcium Sensing Receptor/CASR Antibody Picoband®
Question
We are currently using anti-Calcium Sensing Receptor/CASR antibody PB9924 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The anti-Calcium Sensing Receptor/CASR antibody (PB9924) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-04
