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Product Info Summary
| SKU: | A02819-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Caspr2/CNTNAP2 Antibody Picoband®
SKU/Catalog Number
A02819-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspr2/CNTNAP2 Antibody Picoband® catalog # A02819-2. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Caspr2/CNTNAP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02819-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Caspr2/CNTNAP2 recombinant protein (Position: N363-E1159).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02819-2 is reactive to CNTNAP2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
170 kDa
Calculated molecular weight
96950 MW
Background of Caspr2
Contactin-associated protein-like 2 is a protein that in humans is encoded by the CNTNAP2 gene. This gene encodes a member of the neurexin family which functions in the vertebrate nervous system as cell adhesion molecules and receptors. This protein, like other neurexin proteins, contains epidermal growth factor repeats and laminin G domains. In addition, it includes an F5/8 type C domain, discoidin/neuropilin- and fibrinogen-like domains, thrombospondin N-terminal-like domains and a putative PDZ binding site. This protein is localized at the juxtaparanodes of myelinated axons, and mediates interactions between neurons and glia during nervous system development and is also involved in localization of potassium channels within differentiating axons. This gene encompasses almost 1.5% of chromosome 7 and is one of the largest genes in the human genome. It is directly bound and regulated by forkhead box protein P2, a transcription factor related to speech and language development. This gene has been implicated in multiple neurodevelopmental disorders, including Gilles de la Tourette syndrome, schizophrenia, epilepsy, autism, ADHD and intellectual disability.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02819-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Caspr2/CNTNAP2 using anti-Caspr2/CNTNAP2 antibody (A02819-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspr2/CNTNAP2 antigen affinity purified polyclonal antibody (Catalog # A02819-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspr2/CNTNAP2 at approximately 170 kDa. The expected band size for Caspr2/CNTNAP2 is at 170 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CNTNAP2 (Source: Uniprot.org, NCBI)
Gene Name
CNTNAP2
Full Name
Contactin-associated protein-like 2
Weight
96950 MW
Superfamily
neurexin family
Alternative Names
E3 SUMO-protein ligase PIAS3; Protein inhibitor of activated STAT protein 3; PIAS3 CNTNAP2 AUTS15, CASPR2, CDFE, NRXN4, PTHSL1 contactin associated protein 2 contactin-associated protein-like 2|cell recognition molecule Caspr2|contactin associated protein like 2|homolog of Drosophila neurexin IV
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CNTNAP2, check out the CNTNAP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CNTNAP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspr2/CNTNAP2 Antibody Picoband® (A02819-2)
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