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Product Info Summary
| SKU: | M00080-5 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Chicken, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IP, IF, IHC, WB |
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Product info
Product Name
Anti-Caspase 9 CASP9 Monoclonal Antibody
SKU/Catalog Number
M00080-5
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Caspase 9 CASP9 Monoclonal Antibody catalog # M00080-5. Tested in IP, IF, IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase 9 CASP9 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00080-5)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
43891
Isotype
IgG1
Immunogen
Synthetic Peptide of Caspase 9
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00080-5 is reactive to CASP9 in Chicken, Human, Mouse, Rat
Reconstitution
Calculated molecular weight
46281 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00080-5 is guaranteed for IP, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-5000
IF 1:200
IHC 1:50-300
IP 1:200
Validation Images & Assay Conditions
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Western blot (WB) analysis of Caspase 9 monoclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
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Immunohistochemical analysis of human-lung tissue. Anti-Caspase 9 at 1:200 (4°C, overnight). Antigen retrieval-Sodium Citrate pH6 (>98°C, 20min). Secondary-1:200 (room temp, 30min). Negative control -Secondary only.
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Immunohistochemical analysis of human stomach cancer tissue. Anti-Caspase 9 at 1:200 (4°C, overnight). Antigen retrieval-Sodium Citrate pH6 (>98°C, 20min). Secondary-1:200 (room temp, 30min). Negative control -Secondary only.
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Immunohistochemical analysis of mouse brain tissue. Anti-Caspase 9 at 1:200 (4°C, overnight). Antigen retrieval-Sodium Citrate pH6 (>98°C, 20min). Secondary-1:200 (room temp, 30min). Negative control -Secondary only.
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Immunohistochemical analysis of mouse kidney tissue. Anti-Caspase 9 at 1:200 (4°C, overnight). Antigen retrieval-Sodium Citrate pH6 (>98°C, 20min). Secondary-1:200 (room temp, 30min). Negative control -Secondary only.
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Immunohistochemical analysis of rat kidney tissue. Anti-Caspase 9 at 1:200 (4°C, overnight). Antigen retrieval-Sodium Citrate pH6 (>98°C, 20min). Secondary-1:200 (room temp, 30min). Negative control -Secondary only.
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Immunohistochemical analysis of rat lung tissue. Anti-Caspase 9 at 1:200 (4°C, overnight). Antigen retrieval-Sodium Citrate pH6 (>98°C, 20min). Secondary-1:200 (room temp, 30min). Negative control -Secondary only.
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Immunohistochemical analysis of rat spleen tissue. M00080-5 was diluted at 1:200 (4°C, overnight). Antigen retrieval-Sodium Citrate pH6(>98°C, 20min). Secondary-1:200(room temp, 30min). Negative control -Secondary only.
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Immunoprecipitation (IP) analysis: 1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200Western Blot (WB) analysis of HeLa diluted at 1) 1:2000, 2) 1:5000
Protein Target Info & Infographic
Gene/Protein Information For CASP9 (Source: Uniprot.org, NCBI)
Gene Name
CASP9
Full Name
Caspase-9
Weight
46281 MW
Superfamily
peptidase C14A family
Alternative Names
Caspase-9;CASP-9;3.4.22.62 CASP9 APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56 caspase 9 caspase-9|ICE-like apoptotic protease 6|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9, apoptosis-related cysteine peptidase|protein phosphatase 1, regulatory subunit 56
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP9, check out the CASP9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase 9 CASP9 Monoclonal Antibody (M00080-5)
Hello CJ!
M00080-5 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yue W,Cunlin G,Lu H,Yuanqing Z,Yanjun T,Qiong W. Neuroprotective effect of intermittent hypobaric hypoxia preconditioning on cerebral ischemia/reperfusion in rats. Int J Clin Exp Pathol.2020 Nov 1;13(11):2860-2869.PMID:33284899;PMCID:PMC7716138.
Species: Rat
M00080-5 usage in article: APP:WB, SAMPLE:NEURONS, DILUTION: 1:1500
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