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Product Info Summary
| SKU: | M00334-8 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Caspase-3 Rabbit Monoclonal Antibody, Clone#RM250
SKU/Catalog Number
M00334-8
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Caspase-3 Rabbit Monoclonal Antibody, Clone#RM250 (Catalog # M00334-8). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-3 Rabbit Monoclonal Antibody, Clone#RM250 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00334-8)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM250
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to residues within human Caspase-3 subunit p17
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human full length caspase-3 (35 kDa) and the large fragment of caspase-3 resulting from cleavage (17 kDa).
Reactive Species
M00334-8 is reactive to CASP3 in Human
Reconstitution
Calculated molecular weight
31608 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00334-8 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:1000-1:2500 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of Jurkat cell lysate, untreated or treated with etoposide, using Anti-Caspase-3 RM250 at a 1:1000 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Caspase-3 RM250 at a 1:2500 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CASP3 (Source: Uniprot.org, NCBI)
Gene Name
CASP3
Full Name
Caspase-3
Weight
31608 MW
Superfamily
peptidase C14A family
Alternative Names
Caspase-3;CASP-3;3.4.22.56;Apopain;Cysteine protease CPP32;CPP-32;Protein Yama;SREBP cleavage activity 1;SCA-1;Caspase-3 subunit p17;Caspase-3 subunit p12;CASP3;CPP32; CASP3 CPP32, CPP32B, SCA-1 caspase 3 caspase-3|CASP-3|CPP-32|PARP cleavage protease|SREBP cleavage activity 1|apopain|caspase 3, apoptosis-related cysteine peptidase|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3|protein Yama
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP3, check out the CASP3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-3 Rabbit Monoclonal Antibody, Clone#RM250 (M00334-8)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Caspase-3 Rabbit Monoclonal Antibody, Clone#RM250
Question
We want to test anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
The products we sell, including anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-11
Question
We have tried in the past anti-Caspase-3 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
I have checked the website and datasheets of our anti-Caspase-3 Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2020-02-07
Question
Is a blocking peptide available for product anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 (M00334-8)?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
We do provide the blocking peptide for product anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 (M00334-8). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-03
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-23
Question
My boss were happy with the WB result of your anti-Caspase-3 Rabbit Monoclonal antibody, Clone
S. Carter
Verified customer
Asked: 2019-12-09
Answer
Based on literature, cervix carcinoma erythroleukemia does express CASP3. Generally CASP3 expresses in cytoplasm. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-12-09
Question
Does anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8 work for IHC with cervix carcinoma erythroleukemia?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
According to the expression profile of cervix carcinoma erythroleukemia, CASP3 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8 will work for IHC with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2019-12-06
Question
Is there a BSA free version of anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8 available?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-28
Question
Will M00334-8 anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
You can see on the product datasheet, M00334-8 anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-23
Question
We have been able to see staining in human tongue. What should we do? Is anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 supposed to stain tongue positively?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
From what I have seen in literature tongue does express CASP3. From what I have seen in Uniprot.org, CASP3 is expressed in jejunal mucosa, t-cell, tongue, lymph, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Lymph, Pubmed ID: 15489334
T-cell, Pubmed ID: 7983002, 7774019
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-15
Question
I see that the anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-23
Question
I have a question about product M00334-8, anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00334-8 anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-18
Question
I was wanting to use your anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 for IHC for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2017-10-23
Answer
You can see on the product datasheet, M00334-8 anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-23
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-07-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-03
Question
Is this M00334-8 anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 reactive to the isotypes of CASP3?
Verified Customer
Verified customer
Asked: 2017-06-02
Answer
The immunogen of M00334-8 anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 is A synthetic peptide corresponding to residues within human Caspase-3 subunit p17. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-02
Question
We are currently using anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 M00334-8 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
L. Taylor
Verified customer
Asked: 2016-03-04
Answer
The anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 (M00334-8) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-03-04
Question
I am looking for using your anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 for luteolysis studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.
B. Miller
Verified customer
Asked: 2014-03-05
Answer
I appreciate your inquiry. This M00334-8 anti-Caspase-3 Rabbit Monoclonal antibody, Clone#RM250 is tested on jurkat cell lysate, breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-03-05
