Anti-Caspase-10 CASP10 Antibody

Product Name

Anti-Caspase-10 CASP10 Antibody

View all Caspase-10/FLICE2 Antibodies

SKU/Catalog Number

A02190

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Caspase-10 CASP10 Antibody (Catalog # A02190). Tested in ELISA, WB, IF, ICC applications. This antibody reacts with Human.

Storage & Handling

Caspase-10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Caspase-10 CASP10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02190)

Host

Rabbit

Contents

Caspase-10 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Caspase-10 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human Caspase-10. The immunogen is located within amino acids 240 - 290 of Caspase-10.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Antibody only recognizes FLICE2 form of caspase-10.

Reactive Species

A02190 is reactive to CASP10 in Human

Applications

A02190 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

58951 MW

Background of Caspase-10/FLICE2

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch4 and renamed as caspase-10. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is therefore involved in the CD95 and TNFR1 induced apoptosis. Caspase-10 cleaves and activates caspase-3, -4, -6, -7, -8 and -9, which causes the proteolytic cleavage of many key proteins such as PARP. Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death. Caspase-10 is expressed in many tissues and cell lines.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Caspase-10 antibody can be used for detection of caspase-10 by Western blot 1 μg/mL. A 59 kDa band can be detected.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of caspase-10 in HeLa (H)

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Immunofluorescence of Casp10 in Hela cells with Caspase 10 antibody at 20 μg/mL.

Green: Caspase-10 Antibody (A02190)
Blue: DAPI staining

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Immunocytochemistry of Casp10 in Hela cells with Caspase 10 antibody at 5 μg/mL.

Gene/Protein Information For CASP10 (Source: Uniprot.org, NCBI)

Alternative Names

Apoptotic protease Mch-4; CASP10; CASP-10; caspase 10, apoptosis-related cysteine peptidase; caspase 10, apoptosis-related cysteine protease; Caspase10; Caspase-10; EC 3.4.22.63; FADD-like ICE2; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE2; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; Mch4; MCH4ALPS2 CASP10 ALPS2, FLICE-2, FLICE2, MCH4 caspase 10 caspase-10|CASP-10|FADD-like ICE2|FAS-associated death domain protein interleukin-1B-converting enzyme 2|ICE-like apoptotic protease 4|apoptotic protease MCH-4|caspase 10 apoptosis-related cysteine peptidase|caspase 10, apoptosis-related cysteine protease|interleukin-1B-converting enzyme 2

For more info on CASP10, check out the CASP10 Infographic

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In this infographic, you will see the following information for CASP10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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