Anti-Caspase 10 CASP10 Antibody

Product Name

Anti-Caspase 10 CASP10 Antibody

View all Caspase-10/FLICE2 Antibodies

SKU/Catalog Number

A02190-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Caspase 10 CASP10 Antibody catalog # A02190-1. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Caspase 10 CASP10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02190-1)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Arrestin-beta-1 around the phosphorylation site of S412.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02190-1 is reactive to CASP10 in Human, Mouse, Rat

Applications

A02190-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

58951 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blotting validation for Anti-Caspase 10 CASP10 Antibody A02190-1

Western blot (WB) analysis of Caspase 10 polyclonal antibody at 1:500 dilution
Lane1:HEK293T cell lysate
Lane2:NIH-3T3 cell lysate
Lane3:H9C2 cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Click image to see more details

Figure 2. Western blotting validation for Anti-Caspase 10 CASP10 Antibody A02190-1

Western blot (WB) analysis of Caspase 10 pAb at 1:500 dilution
Lane1:H9C2 whole cell lysate(40ug)
Lane2:HepG2 whole cell lysate(40ug)
Lane3:CT26 whole cell lysate(40ug)
Lane4:L02 whole cell lysate(40ug)
Lane5:SK-OVCAR3 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For CASP10 (Source: Uniprot.org, NCBI)

Alternative Names

Apoptotic protease Mch-4; CASP10; CASP-10; caspase 10, apoptosis-related cysteine peptidase; caspase 10, apoptosis-related cysteine protease; Caspase10; Caspase-10; EC 3.4.22.63; FADD-like ICE2; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE2; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; Mch4; MCH4ALPS2 CASP10 ALPS2, FLICE-2, FLICE2, MCH4 caspase 10 caspase-10|CASP-10|FADD-like ICE2|FAS-associated death domain protein interleukin-1B-converting enzyme 2|ICE-like apoptotic protease 4|apoptotic protease MCH-4|caspase 10 apoptosis-related cysteine peptidase|caspase 10, apoptosis-related cysteine protease|interleukin-1B-converting enzyme 2

For more info on CASP10, check out the CASP10 Infographic

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In this infographic, you will see the following information for CASP10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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