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Product Info Summary
| SKU: | PA1522 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Caspase-1(P10)/CASP1 Antibody Picoband®
SKU/Catalog Number
PA1522
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase-1 (P10)/CASP1 Antibody catalog # PA1522. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-1(P10)/CASP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1522)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-1(P10).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1522 is reactive to CASP1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
45159 MW
Background of Caspase-1
Caspase 1, apoptosis-related cysteine protease, is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, IL18, and IL33 precursor proteins. Caspase 1 was purified ICE from the cytosol of the THP. human monocytic cell line and found that the active protease was made up of 2 peptides, which they called p20 and p10 based on their apparent molecular masses by SDS-PAGE. It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. The Caspase1 gene consists of 10 exons spanning at least 10.6 kb. The Caspase 1 gene is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1522 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: MCF-7 Whole Cell, HELA Whole Cell, SW620 Whole Cell
IHC: Human Mammary Cancer Tissue
Validation Images & Assay Conditions
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Anti-Caspase-1(P10) antibody, PA1522, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Anti-Caspase-1(P10) antibody, PA1522, Western blotting
All lanes: Anti CASP1(P10) (PA1522) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD
Protein Target Info & Infographic
Gene/Protein Information For CASP1 (Source: Uniprot.org, NCBI)
Gene Name
CASP1
Full Name
Caspase-1
Weight
45159 MW
Superfamily
peptidase C14A family
Alternative Names
Caspase-1;CASP-1;3.4.22.36;Interleukin-1 beta convertase;IL-1BC;Interleukin-1 beta-converting enzyme;ICE;IL-1 beta-converting enzyme;p45;Caspase-1 subunit p20;Caspase-1 subunit p10;CASP1;IL1BC, IL1BCE; CASP1 ICE, IL1BC, P45 caspase 1 caspase-1|CASP1 nirs variant 1|IL-1 beta-converting enzyme|IL1B-convertase|caspase 1, apoptosis-related cysteine peptidase|caspase-1 isoform alpha|interleukin 1, beta, convertase|interleukin 1-B converting enzyme
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP1, check out the CASP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-1(P10)/CASP1 Antibody Picoband® (PA1522)
Hello CJ!
PA1522 has been cited in 22 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Peng Yuan,Xifeng Sun,Xiao Liu,Georg Hutterer,Karl Pummer,Boris Hager,Zhangqun Ye,Zhiqiang Chen,Kaempferol alleviates calcium oxalate crystal-induced renal injury and crystal deposition via regulation of the AR/NOX2 signaling pathway,Phytomedicine,2021,153555,ISSN 0944-7113,https://doi.org/10.1016/j.phymed.2021.153555.
Species: Human,Mouse
PA1522 usage in article: APP:WB, SAMPLE:HK-2 CELL, DILUTION:1:200
Ding Y, Zou J, Li Z, Tian J, Abdelalim S, Du F, She R, Wang D, Tan C, Wang H, Chen W, Lv D, Chang L. Plos One. 2011;6(5):E20008. Doi: 10.1371/Journal.Pone.0020008. Epub 2011 May 23. Study Of Histopathological And Molecular Changes Of Rat Kidney Un...
Zhang D, Yan H, Hu Y, Zhuang Z, Yu Z, Hang C. J Stroke Cerebrovasc Dis. 2015 May;24(5):972-9. Doi: 10.1016/J.Jstrokecerebrovasdis.2014.12.019. Epub 2015 Mar 23. Increased Expression Of Nlrp3 Inflammasome In Wall Of Ruptured And Unruptured Human Ce...
Peng K, Liu L, Wei D, Lv Y, Wang G, Xiong W, Wang X, Altaf A, Wang L, He D, Wang H, Qu P. Int J Mol Med. 2015 May;35(5):1179-88. Doi: 10.3892/Ijmm.2015.2129. Epub 2015 Mar 9. P2X7R Is Involved In The Progression Of Atherosclerosis By Promoting Nlr...
Zhao Y, Wu S, Wu J, Jia P, Gao S, Yan X, Wang Y. Cancer Biol Ther. 2011 Jan 1;11(1):95-107. Doi: 10.4161/Cbt.11.1.13960. Introduction Of Hypoxia-Targeting P53 Fusion Protein For The Selective Therapy Of Non-Small Cell Lung Cancer.
Liu N, Chen T, Wang X, Yang D, Xue B, Zhu H. Febs Lett. 2015 Apr 2;589(8):897-903. Doi: 10.1016/J.Febslet.2015.02.026. Epub 2015 Mar 3. Msi1 Confers Resistance To Trail By Activating Erk In Liver Cancer Cells.
Shu Hf, Yu Sx, Zhang Cq, Liu Sy, Wu Kf, Zang Zl, Yang H, Zhou Sw, Yin Q. Brain Dev. 2013 Mar;35(3):252-60. Doi: 10.1016/J.Braindev.2012.04.007. Epub 2012 May 28. Expression Of Trpv1 In Cortical Lesions From Patients With Tuberous Sclerosis Complex...
Wang Xf, Qu Xq, Zhang Tt, Zhang Jf. Exp Ther Med. 2015 Apr;9(4):1283-1291. Epub 2015 Feb 6. Testosterone Suppresses Ventricular Remodeling And Improves Left Ventricular Function In Rats Following Myocardial Infarction.
Li M, Peng J, Song Y, Liang H, Mei Y, Fang Y. J Huazhong Univ Sci Technolog Med Sci. 2012 Oct;32(5):746-9. Doi: 10.1007/S11596-012-1028-0. Epub 2012 Oct 18. Electro-Acupuncture Combined With Transcranial Magnetic Stimulation Improves Learning And ...
Wu Z, Wang L, Hou S. Neural Regen Res. 2012 Jul 5;7(19):1493-7. Doi: 10.3969/J.Issn.1673-5374.2012.19.008. Alpha B-Crystallin Improved Survival Of Retinal Ganglion Cells In A Rat Model Of Acute Ocular Hypertension.
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Caspase-1(P10)/CASP1 Antibody Picoband®
Question
We are currently using anti-Caspase-1(P10)/CASP1 antibody PA1522 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
The anti-Caspase-1(P10)/CASP1 antibody (PA1522) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-13
Question
We purchased anti-Caspase-1(P10)/CASP1 antibody for IHC on macrophage in a previous experiment. I am using human, and We are going to use the antibody for WB next. Our lab want to know about examining macrophage as well as leukocyte in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
I have checked the website and datasheets of our anti-Caspase-1(P10)/CASP1 antibody and it appears that PA1522 has been tested on human in both IHC and WB. Thus PA1522 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-10
Question
We have observed staining in human testis. Are there any suggestions? Is anti-Caspase-1(P10)/CASP1 antibody supposed to stain testis positively?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
From literature testis does express CASP1. From Uniprot.org, CASP1 is expressed in leukocyte, testis, macrophage, among other tissues. Regarding which tissues have CASP1 expression, here are a few articles citing expression in various tissues:
Macrophage, Pubmed ID: 20148899
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-09
Question
Has PA1522 and PA1440 been tested for IHC-F on human samples?
Verified customer
Asked: 2019-03-29
Answer
Unfortunately, we have not tested the Anti-Caspase-1(P10)/CASP1 Antibody (PA1522) and Anti-Caspase-1(P20)/CASP1 Antibody (PA1440) for IHC-F on human samples. So, we do not know if they will work for IHC-F.
Boster Scientific Support
Answered: 2019-04-01
Question
We were content with the WB result of your anti-Caspase-1(P10)/CASP1 antibody. However we have seen positive staining in leukocyte cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP1 supposed to be expressed?
T. Jackson
Verified customer
Asked: 2018-11-01
Answer
From literature, leukocyte does express CASP1. Generally CASP1 expresses in cytoplasm. Regarding which tissues have CASP1 expression, here are a few articles citing expression in various tissues:
Macrophage, Pubmed ID: 20148899
Testis, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-11-01
Question
My lab would like using your anti-Caspase-1(P10)/CASP1 antibody for activation of cysteine-type endopeptidase activity involved in apoptotic process studies. Has this antibody been tested with western blotting on sw620 whole cell lysate? We would like to see some validation images before ordering.
M. Edwards
Verified customer
Asked: 2016-08-09
Answer
Thank you for your inquiry. This PA1522 anti-Caspase-1(P10)/CASP1 antibody is tested on mammary cancer tissue, hela whole cell lysate, sw620 whole cell lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-08-09
