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Facts about Caspase-1.
Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
Human | |
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Gene Name: | CASP1 |
Uniprot: | P29466 |
Entrez: | 834 |
Belongs to: |
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peptidase C14A family |
CASP1; CASP-1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta; caspase 1, apoptosis-related cysteine protease (interleukin 1, beta; Caspase1; Caspase-1; EC 3.4.22; EC 3.4.22.36; ICE; ICEP45; IL-1 beta-converting enzyme; IL1BC CASP1 nirs variant 1; IL-1BC; IL1BCE; IL1B-convertase; interleukin 1-B converting enzyme; interleukin 1-beta convertase; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45
Mass (kDA):
45.159 kDA
Human | |
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Location: | 11q22.3 |
Sequence: | 11; NC_000011.10 (105025443..105035591, complement) |
Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
Cytoplasm. Cell membrane.
You might be in search of an anti-body that is new and you are unsure if Randox Life Sciences or Boster Bio are the right choice for your needs. Both companies are in the business of creating antibodies for human immunology research However, which one is the most appropriate to your needs? There are numerous advantages to both products. But which one is best suited for your specific project? We've created an overview of the benefits for each company, so that you can decide which one to make use of based on the information you've gathered.
The pGEX-4T-3 bacterial expression plasmid, containing the p30 portion of caspase, was designed to express human caspase. The pGEX-4T-3 expression plasmid has the XhoI restriction that was used for CASPp30 DNAs. The CASPp30 DNAs were cloned to pGEX-4T-3 plasmids and digested by EcoRV or XhoI.
To prevent cross-reactivity between CASP1 subfamilies The primers were CASP1-specific. The primers were selected because of the mismatch between the mRNAs. For the CASP1 primer, the mismatch was 61-75%. However the reverse primer, CASP1-reverse, had 75-100% mismatch when in comparison to the other CASP subfamily members.
GAPDH (and Casp1) were amplified using an established PCR procedure in order to carry out the CASP1 test. GAPDH and CASP1 primers had attB flanking locations. The PCR products were utilized in immunostaining experiments with Boster bioreagents. To compare the CASP1 marker to other genes, it's useful to examine the CASP1 gene with IL-1b or IL-18.
The CASP1 marker is a basic element of a molecular diagnosis test. The marker is able to help determine the possibility of developing certain diseases or determining if a certain treatment is working. The CASP1 marker is used for a variety purposes, such as the monitoring of disease progression, prognostication and approval by the FDA. Randox Biosciences offers over 320 biomarker tests for clinical use that can be customized.
Antibodies to the CASP1 protein are available from several sources. They are distinguished by their affinity to the proteins that they target. They can be conjugated using a variety of reagents, including fluorescent dyes and biotin. The Boster Bio guide also contains instructions for preparing samples for immunostaining. This guide provides step-by-step procedures and product datasheets. This guide will help you select the best reagents for the analysis of CASP1 expression in your samples.
PMID: 1574116 by Thornberry N.A., et al. A novel heterodimeric cysteine protease is required for interleukin-1 beta processing in monocytes.
PMID: 1373520 by Cerretti D.P., et al. Molecular cloning of the interleukin-1 beta converting enzyme.
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