Anti-CARS Antibody Picoband®

CARS1 antibody

Boster Bio Anti-CARS Antibody Picoband® catalog # A00259-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A00259-1
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-CARS Antibody Picoband®

View all CARS1 Antibodies

SKU/Catalog Number

A00259-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CARS Antibody Picoband® catalog # A00259-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CARS Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00259-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human CARS recombinant protein (Position: D510-Q748).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00259-1 is reactive to CARS in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

85 kDa

Calculated molecular weight

85473 MW

Background of CARS1

This CARS gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A00259-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml

Positive Control

WB: human Hela cell, human COLO-320 cell, human A549 cell, human PANC-1 cell, human 22RV1 cell
IHC: human placenta tissue
ICC/IF: U20S cell
FCM: A431 cell

Validation Images & Assay Conditions

Gene/Protein Information For CARS (Source: Uniprot.org, NCBI)

Gene Name

CARS

Full Name

Cysteine--tRNA ligase, cytoplasmic

Weight

85473 MW

Superfamily

class-I aminoacyl-tRNA synthetase family

Alternative Names

Cysteine--tRNA ligase, cytoplasmic;6.1.1.16;Cysteinyl-tRNA synthetase;CysRS;CARS; CARS1 CARS, CYSRS, MCDDBH, MDBH, MGC:11246 cysteinyl-tRNA synthetase 1 cysteine--tRNA ligase, cytoplasmic|cysteine tRNA ligase 1, cytoplasmic|cysteine translase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CARS, check out the CARS Infographic

CARS infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CARS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-CARS Antibody Picoband®

Question

We are currently using anti-CARS antibody A00259-1 for mouse tissue, and we are well pleased with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

The anti-CARS antibody (A00259-1) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-22

Question

We have observed staining in rat brain. What should we do? Is anti-CARS antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2020-01-16

Answer

According to literature brain does express CARS. According to Uniprot.org, CARS is expressed in endothelial cell, brain, testis, cervix, lung, cervix carcinoma, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have CARS expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7987009
Cervix, Pubmed ID: 17974005
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Testis, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-01-16

Question

Is there a BSA free version of anti-CARS antibody A00259-1 available?

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CARS antibody A00259-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-30

Question

Please see the WB image, lot number and protocol we used for liver using anti-CARS antibody A00259-1. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-05

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-05

Question

I was wanting to use your anti-CARS antibody for Flow Cytometry for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

As indicated on the product datasheet, A00259-1 anti-CARS antibody has been validated for ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-23

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CARS antibody A00259-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-04-29

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-04-29

Question

I see that the anti-CARS antibody A00259-1 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-01-02

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-01-02

Question

I am looking for to test anti-CARS antibody A00259-1 on human liver for research purposes, then I may be interested in using anti-CARS antibody A00259-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-05-23

Answer

The products we sell, including anti-CARS antibody A00259-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-05-23

Question

I am looking for using your anti-CARS antibody for cysteinyl-trna aminoacylation studies. Has this antibody been tested with western blotting on a431 cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-02-20

Answer

Thank you for your inquiry. This A00259-1 anti-CARS antibody is tested on human hela, a431 cells. It is guaranteed to work for ELISA, Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-02-20

Question

I have a question about product A00259-1, anti-CARS antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-02-07

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00259-1 anti-CARS antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-02-07

Question

Will anti-CARS antibody A00259-1 work for Flow Cytometry with liver?

Verified Customer

Verified customer

Asked: 2017-10-18

Answer

According to the expression profile of liver, CARS is highly expressed in liver. So, it is likely that anti-CARS antibody A00259-1 will work for Flow Cytometry with liver.

Boster Scientific Support

Answered: 2017-10-18

Question

Is a blocking peptide available for product anti-CARS antibody (A00259-1)?

J. Bhatt

Verified customer

Asked: 2017-06-05

Answer

We do provide the blocking peptide for product anti-CARS antibody (A00259-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-06-05

Question

Is this A00259-1 anti-CARS antibody reactive to the isotypes of CARS?

L. Jones

Verified customer

Asked: 2017-03-28

Answer

The immunogen of A00259-1 anti-CARS antibody is E. coli-derived human CARS recombinant protein (Position: D510-Q748). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-03-28

Question

Would A00259-1 anti-CARS antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

D. Johnson

Verified customer

Asked: 2016-12-30

Answer

It shows on the product datasheet, A00259-1 anti-CARS antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2016-12-30

Question

My boss were well pleased with the WB result of your anti-CARS antibody. However we have been able to see positive staining in brain cytoplasm. using this antibody. Is that expected? Could you tell me where is CARS supposed to be expressed?

G. Yang

Verified customer

Asked: 2015-02-12

Answer

From literature, brain does express CARS. Generally CARS expresses in cytoplasm. Regarding which tissues have CARS expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 7987009
Cervix, Pubmed ID: 17974005
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 15489334
Testis, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2015-02-12

Question

We ordered your anti-CARS antibody for ICC on lung in a previous experiment. I am using mouse, and We want to use the antibody for WB next. I am interested in examining lung as well as testis in our next experiment. Could give a recommendation on which antibody would work the best for WB?

L. Edwards

Verified customer

Asked: 2013-02-12

Answer

I viewed the website and datasheets of our anti-CARS antibody and it seems that A00259-1 has been validated on mouse in both ICC and WB. Thus A00259-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-02-12

Order DetailsPrice
A00259-1

100μg

$370
A00259-1-10ug

10μg sample (liquid)

$99
A00259-1-Biotin

100 μg Biotin conjugated

$570
A00259-1-Cy3

100 μg Cy3 conjugated

$570
A00259-1-Dylight488

100 μg Dylight488 conjugated

$570
A00259-1-Dylight550

100 μg Dylight550 conjugated

$570
A00259-1-Dylight594

100 μg Dylight594 conjugated

$570
A00259-1-FITC

100 μg FITC conjugated

$570
A00259-1-HRP

100 μg HRP conjugated

$570
A00259-1-APC

100 μg APC conjugated

$670
A00259-1-PE

100 μg PE conjugated

$670
A00259-1-iFluor647

100 μg iFluor647 conjugated

$670
A00259-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A00259-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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