Anti-CARD9 Antibody (N-term)

Product Name

Anti-CARD9 Antibody (N-term)

View all CARD9 Antibodies

SKU/Catalog Number

M01410

Size

50 µl

Description

Boster Bio Anti-CARD9 Antibody (N-term) (Catalog # M01410). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-CARD9 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01410)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

2156CT20.4.85

Isotype

IgG1,κ

Immunogen

This CARD9 antibody is generated from a mouse immunized with a recombinant protein from human CARD9.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01410 is reactive to CARD9 in Human

Reconstitution

Calculated molecular weight

62241 Da

Background of CARD9

Adapter protein that plays a key role in innate immune response to a number of intracellular pathogens, such as C.albicans and L.monocytogenes. Is at the crossroads of ITAM- tyrosine kinase and the Toll-like receptors (TLR) and NOD2 signaling pathways. Probably controls various innate immune response pathways depending on the intracellular pathogen. In response to L.monocytogenes infection, acts by connecting NOD2 recognition of peptidoglycan to downstream activation of MAP kinases (MAPK) without activating NF-kappa-B. Also involved in activation of myeloid cells via classical ITAM-associated receptors and TLR: required for TLR-mediated activation of MAPK, while it is not required for TLR-induced activation of NF-kappa-B (By similarity). Controls CLEC7A (dectin-1)-mediated myeloid cell activation induced by the yeast cell wall component zymosan, leading to cytokine production and innate anti-fungal immunity: acts by regulating BCL10-MALT1-mediated NF-kappa-B activation pathway. Activates NF-kappa-B via BCL10. In response to the hyphal form of C.albicans, mediates CLEC6A (dectin-2)-induced I-kappa-B kinase ubiquitination, leading to NF-kappa-B activation via interaction with BCL10. In response to fungal infection, may be required for the development and subsequent differentiation of interleukin 17-producing T helper (TH-17) cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01410 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500

Validation Images & Assay Conditions

Gene/Protein Information For CARD9 (Source: Uniprot.org, NCBI)

Alternative Names

Caspase recruitment domain-containing protein 9, hCARD9, CARD9 CARD9 CANDF2, hCARD9 caspase recruitment domain family member 9 caspase recruitment domain-containing protein 9

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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