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Product Info Summary
| SKU: | MA1081 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)
SKU/Catalog Number
MA1081
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) catalog # MA1081. Tested in IHC, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1081)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2
Isotype
Mouse IgG2a/IgG1
Immunogen
mixture of several monoclonal cytokeratin clones.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1081 is reactive to KRT1 in Human, Rat
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
45-60 kDa
Calculated molecular weight
66039 MW
Background of KRT1
Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1081 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.1-0.5μg/ml, Human, rat, By Heat
Immunohistochemistry (Frozen Section), 0.1-0.5μg/ml, Human, rat, -
Western blot, 1-2μg/ml, Human, rat
Positive Control
WB: human NCL-H460 whole cellhuman Hela whole cellhuman A431 whole cell
IHC: Human Placenta tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of PCK using anti-PCK antibody (MA1081).
PCK was detected in a paraffin-embedded section of Human Placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 0.5 μg/ml mouse anti-PCK Antibody (MA1081) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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Figure 2. Western blot analysis of PCK using anti-PCK antibody (MA1081).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human NCL-H460 whole cell lysates.
Lane 2: human Hela whole cell lysates.
Lane 3: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PCK antigen affinity purified monoclonal antibody (Catalog # MA1081) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for PCK at approximately 45-60 kDa. The expected band size for PCK is at 66 kDa.
Protein Target Info & Infographic
Gene/Protein Information For KRT1 (Source: Uniprot.org, NCBI)
Gene Name
KRT1
Full Name
Keratin, type II cytoskeletal 1
Weight
66039 MW
Superfamily
intermediate filament family
Alternative Names
Keratin, type II cytoskeletal 1;67 kDa cytokeratin;Cytokeratin-1;CK-1;Hair alpha protein;Keratin-1;K1;Type-II keratin Kb1;KRT1;KRTA; KRT1 CK1, EHK, EHK1, EPPK, K1A, NEPPK, KRT1 keratin 1 keratin, type II cytoskeletal 1|67 kDa cytokeratin|CK-1|cytokeratin 1|epidermolytic hyperkeratosis 1|hair alpha protein|keratin 1, type II|type-II keratin Kb1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT1, check out the KRT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) (MA1081)
Hello CJ!
MA1081 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A metanephric adenoma of the kidney associated with polycythemia: A case report
Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage
Bai J, Liu H, Li D, Cui L, Wu X. Plos One. 2012;7(11):E49673. Doi: 10.1371/Journal.Pone.0049673. Epub 2012 Nov 20. Duodenum Clamping Trauma Induces Significant Postoperative Intraperitoneal Adhesions On A Rat Model.
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Customer Q&As
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7 Customer Q&As for Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)
Question
Is a blocking peptide available for product anti-PCK antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) (MA1081)?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
We do provide the blocking peptide for product anti-PCK antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) (MA1081). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-02
Question
Would anti-PCK antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) MA1081 work for IHC with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
According to the expression profile of cervix carcinoma, KRT1 is highly expressed in cervix carcinoma. So, it is likely that anti-PCK antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) MA1081 will work for IHC with cervix carcinoma.
Boster Scientific Support
Answered: 2019-05-15
Question
Is there a BSA free version of anti-PCK antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) MA1081 available?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PCK antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) MA1081 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-12-18
Question
Is this MA1081 anti-PCK antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) reactive to the isotypes of KRT1?
A. Banerjee
Verified customer
Asked: 2017-07-03
Answer
The immunogen of MA1081 anti-PCK antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) is mixture of several monoclonal cytokeratin clones. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-03
Question
I have a question about product MA1081, anti-PCK antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Kulkarni
Verified customer
Asked: 2015-04-27
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1081 anti-PCK antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-04-27
Question
We want to test anti-PCK antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) MA1081 on human cervix carcinoma for research purposes, then I may be interested in using anti-PCK antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) MA1081 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Roberts
Verified customer
Asked: 2014-05-06
Answer
The products we sell, including anti-PCK antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) MA1081, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-05-06
Question
Does anti-PCK antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) MA1081 work on horse WB with skin of abdomen?
J. Zhang
Verified customer
Asked: 2013-10-21
Answer
Our lab technicians have not validated anti-PCK antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) MA1081 on horse. You can run a BLAST between horse and the immunogen sequence of anti-PCK antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) MA1081 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse skin of abdomen in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-10-21
