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Product Info Summary
| SKU: | A03404-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-CARD8 Antibody
SKU/Catalog Number
A03404-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CARD8 Antibody (Catalog # A03404-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CARD8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03404-1)
Host
Rabbit
Contents
CARD8 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CARD8 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARD8. The immunogen is located within the last 50 amino acids of CARD8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Two alternatively spliced transcript variants have been observed.
Reactive Species
A03404-1 is reactive to CARD8 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
48933 MW
Background of CARD8
Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis, and caspase-1 and its related proteins ICEBERG and pesudo-ICE. Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein, it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-κB activation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03404-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CARD8 antibody can be used for detection of CARD8 by Western blot at 1 -2 μg/mL. An approximate 45 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CARD8 expression in human lung tissue lysate with CARD8 antibody at (A) 1 and (B) 2 μg/ml.
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Immunocytochemistry of CARD8 in K562 cells with CARD8 antibody at 10 μg/mL.
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Immunofluorescence of CARD8 in K562 cells with CARD8 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CARD8 (Source: Uniprot.org, NCBI)
Gene Name
CARD8
Full Name
Caspase recruitment domain-containing protein 8
Weight
48933 MW
Alternative Names
NDPP, DACAR, DAKAR, NDPP1, TUCAN, CARDINAL, KIAA0955, Caspase recruitment domain-containing protein 8, Apoptotic protein NDPP1 CARD8 CARDINAL, DACAR, DAKAR, NDPP, NDPP1, TUCAN caspase recruitment domain family member 8 caspase recruitment domain-containing protein 8|CARD inhibitor of NF-kappaB-activating ligands|apoptotic protein NDPP1|tumor up-regulated CARD-containing antagonist of CASP9|tumor up-regulated CARD-containing antagonist of caspase nine
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CARD8, check out the CARD8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CARD8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CARD8 Antibody (A03404-1)
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