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Product Info Summary
| SKU: | PA2111 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-NFkB/NFKB1 p105/p50 Antibody Picoband®
SKU/Catalog Number
PA2111
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NFkB/NFKB1 p105/p50 Antibody catalog # PA2111. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NFkB/NFKB1 p105/p50 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2111)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse NFkB/NFKB1 p105/p50, identical to the related rat sequence, and different from the related human sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2111 is reactive to Nfkb1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
105 kDa
Calculated molecular weight
105615 MW
Background of NFKB1
Nuclear factor NF-kappa-B p105 subunit, also called EBP-1 is a protein that in humans is encoded by the NFKB1 gene. By fluorescence in situ hybridization, the gene was assigned to human chromosome 4q24. NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2111 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Liver Tissue, Rat Spleen Tissue, Rat Testis Tissue, HELA Whole Cell, A431 Whole Cell, JURKAT Whole Cell
Validation Images & Assay Conditions
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Anti-NFkB p105/p50 antibody, PA2111, Western blotting
All lanes: Anti NFkB p105/p50 (PA2111) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: Rat Testis Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: A431 Whole Cell Lysate at 40ug
Lane 6: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 50KD
Observed bind size: 105KD
Protein Target Info & Infographic
Gene/Protein Information For Nfkb1 (Source: Uniprot.org, NCBI)
Gene Name
Nfkb1
Full Name
Nuclear factor NF-kappa-B p105 subunit
Weight
105615 MW
Alternative Names
Nuclear factor NF-kappa-B p105 subunit;DNA-binding factor KBF1;EBP-1;NF-kappa-B1 p84/NF-kappa-B1 p98;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1;Nuclear factor NF-kappa-B p50 subunit;Nfkb1; NFKB1 CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1, NF-kappaB, NF-kappabeta, NFKB-p105, NFKB-p50, NFkappaB nuclear factor kappa B subunit 1 nuclear factor NF-kappa-B p105 subunit|DNA-binding factor KBF1|nuclear factor NF-kappa-B p50 subunit|nuclear factor kappa-B DNA binding subunit|nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Nfkb1, check out the Nfkb1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Nfkb1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NFkB/NFKB1 p105/p50 Antibody Picoband® (PA2111)
Hello CJ!
PA2111 has been cited in 12 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
HMGB1, TGF-? and NF-?B are associated with chronic allograft nephropathy
MiR199b Suppresses Expression of Hypoxia-Inducible Factor 1? (HIF-1?) in Prostate Cancer Cells
Hydrogen sulfide attenuates myocardial fibrosis in diabetic rats through the JAK/STAT signaling pathway
Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice
Leng Am, Liu T, Yang J, Cui Jf, Li Xh, Zhu Yn, Xiong T, Zhang G, Chen Y. Cell Biol Int. 2012 Oct 1;36(10):893-9. Doi: 10.1042/Cbi20110473. The Apoptotic Effect And Associated Signalling Of Hsp90 Inhibitor 17-Dmag In Hepatocellular Carcinoma Cells.
Zhao Yj, Wang H, Liu X, Sun M, Kazuhiro H. Mol Med Rep. 2012 Oct;6(4):739-44. Doi: 10.3892/Mmr.2012.1007. Epub 2012 Jul 26. Protective Effects Of Glutamine In A Rat Model Of Endotoxemia.
Xu M, Wang Kn, Wu K, Wang Xp. Gut Liver. 2015 May 23;9(3):411-6. Doi: 10.5009/Gnl14050. Pyrrolidine Dithiocarbamate Inhibits Nuclear Factor ??b And Toll-Like Receptor 4 Expression In Rats With Acute Necrotizing Pancreatitis.
Li Y, Chen Y, Zhang J, Zhu Jf, Liu Zj, Liang Sy, Sun K, Liao Wy, Gong Jp. Am J Surg. 2010 Jan;199(1):35-42. Doi: 10.1016/J.Amjsurg.2008.11.039. Protective Effect Of Glutamine-Enriched Early Enteral Nutrition On Intestinal Mucosal Barrier Injury Af...
Liu Mw, Wang Yh, Qian Cy, Li H. Int J Mol Med. 2014 Dec;34(6):1492-504. Doi: 10.3892/Ijmm.2014.1943. Epub 2014 Sep 23. Xuebijing Exerts Protective Effects On Lung Permeability Leakage And Lung Injury By Upregulating Toll-Interacting Protein Expres...
Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.
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