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Product Info Summary
| SKU: | A03241-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CaMKII alpha/CAMK2A Antibody Picoband®
SKU/Catalog Number
A03241-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CaMKII alpha/CAMK2A Antibody Picoband® catalog # A03241-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CaMKII alpha/CAMK2A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03241-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CaMKII alpha recombinant protein (Position: L392-R457).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03241-1 is reactive to CAMK2A in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa; 60 kDa
Calculated molecular weight
54.088kDa
Background of CAMK2A
MAX (Max protein), also called Myc-associated factor x, is the most conserved dimerization component of the MYC-MAX-MXD1 network of basic helix-loop-helix leucine zipper (bHLHZ) transcription factors that regulate cell proliferation, differentiation, and apoptosis. The conservation of the MAX sequence is particularly high in the bHLHZ domain, which is involved in protein-protein interactions and DNA binding. The MAX gene is located on chromosome 14q23 by fluorescence in situ chromosomal hybridization. Both quasisymmetric heterodimers resemble the symmetric MAX homodimer, albeit with marked structural differences in the coiled-coil leucine zipper regions that explain preferential homo- and heteromeric dimerization of these 3 evolutionarily related DNA-binding proteins. MAX acts as a classic tumor suppressor gene. Normal lymphocytes from patients showed absence of methylation of the MAX promoter and biallelic expression of MAX, which ruled out an imprinting-mediated effect on MAX expression. The ability of these cells to divide, differentiate, and apoptose in the absence of Max demonstrated for the first time that these processes can occur via Max- and possibly Myc-independent mechanisms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03241-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CaMKII alpha using anti-CaMKII alpha antibody (A03241-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CaMKII alpha antigen affinity purified polyclonal antibody (Catalog # A03241-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CaMKII alpha at approximately 50, 60KD. The expected band size for CaMKII alpha is at 54KD.
Protein Target Info & Infographic
Gene/Protein Information For CAMK2A (Source: Uniprot.org, NCBI)
Gene Name
CAMK2A
Full Name
Calcium/calmodulin-dependent protein kinase type II subunit alpha
Weight
54.088kDa
Superfamily
protein kinase superfamily
Alternative Names
Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha CAMK2A CAMKA, CaMKIINalpha, CaMKIIalpha, MRD53, MRT63 calcium/calmodulin dependent protein kinase II alpha calcium/calmodulin-dependent protein kinase type II subunit alpha|CaM kinase II alpha subunit|CaM-kinase II alpha chain|CaMK-II alpha subunit|caM kinase II subunit alpha|caMK-II subunit alpha|calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha|calcium/calmodulin-dependent protein kinase II alpha-B subunit|calcium/calmodulin-dependent protein kinase type II alpha chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAMK2A, check out the CAMK2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAMK2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CaMKII alpha/CAMK2A Antibody Picoband® (A03241-1)
Hello CJ!
A03241-1 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Meng, X.,Fu, M.,Wang, S.,Chen, W.,Wang, J.,& Zhang, N.(2021). Naringin ameliorates memory deficits and exerts neuroprotective effects in a mouse model of Alzheimer‘s disease by regulating multiple metabolic pathways. Molecular Medicine Reports,23,332.https://doi.org/10.3892/mmr.2021.11971
Species: Mouse
A03241-1 usage in article: APP:WB, SAMPLE:HIPPOCAMPUS TISSUE, DILUTION:1:1000
Inhibition of CaMKII%u03B1 in the central nucleus of amygdala attenuates fentanyl-induced hyperalgesia in rats
Anti-Epileptic Effect of Ganoderma Lucidum Polysaccharides by Inhibition of Intracellular Calcium Accumulation and Stimulation of Expression of CaMKII ? in Epileptic Hippocampal Neurons
Li Z, Yin P, Chen J, Jin S, Liu J, Luo F. PLoS One. 2017 May 10;12(5):e0177412. doi: 10.1371/journal.pone.0177412. eCollection 2017. CaMKIIα may modulate fentanyl-induced hyperalgesia via a CeLC-PAG-RVM-spinal cord descending facilitative pain pat...
Sun Z, Niu R, Su K, Wang B, Wang J, Zhang J, Wang J. Arch Toxicol. 2010 May;84(5):353-61. Doi: 10.1007/S00204-009-0508-X. Epub 2010 Jan 6. Effects Of Sodium Fluoride On Hyperactivation And Ca2+ Signaling Pathway In Sperm From Mice: An In Vivo Study.
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Customer Q&As
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1 Customer Q&As for Anti-CaMKII alpha/CAMK2A Antibody Picoband®
Question
We are currently using anti-CaMKII alpha/CAMK2A antibody A03241-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
The anti-CaMKII alpha/CAMK2A antibody (A03241-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-11
