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Product Info Summary
| SKU: | M03241 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-CaMKII alpha CAMK2A Rabbit Monoclonal Antibody
SKU/Catalog Number
M03241
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CaMKII alpha CAMK2A Rabbit Monoclonal Antibody catalog # M03241. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CaMKII alpha CAMK2A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03241)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAFF-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CaMKII alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03241 is reactive to CAMK2A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
54 kDa
Calculated molecular weight
54088 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03241 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IP 1:50
FC 1:100
Positive Control
WB: SH-SY5Y cell, human Hela whole cell, human K562 whole cell, human SH-SY5Y whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DBN1 using anti-DBN1 antibody (M05530).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBN1 antigen affinity purified monoclonal antibody (Catalog # M05530) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DBN1 at approximately 120 kDa. The expected band size for DBN1 is at 71 kDa.
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Western blot analysis of CaMKII alpha expression in SH-SY5Y cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CAMK2A (Source: Uniprot.org, NCBI)
Gene Name
CAMK2A
Full Name
Calcium/calmodulin-dependent protein kinase type II subunit alpha
Weight
54088 MW
Superfamily
protein kinase superfamily
Alternative Names
Calcium/calmodulin-dependent protein kinase type II subunit alpha;CaM kinase II subunit alpha;CaMK-II subunit alpha;2.7.11.17 ;CAMK2A;CAMKA, KIAA0968; CAMK2A CAMKA, CaMKIINalpha, CaMKIIalpha, MRD53, MRT63 calcium/calmodulin dependent protein kinase II alpha calcium/calmodulin-dependent protein kinase type II subunit alpha|CaM kinase II alpha subunit|CaM-kinase II alpha chain|CaMK-II alpha subunit|caM kinase II subunit alpha|caMK-II subunit alpha|calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha|calcium/calmodulin-dependent protein kinase II alpha-B subunit|calcium/calmodulin-dependent protein kinase type II alpha chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAMK2A, check out the CAMK2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAMK2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CaMKII alpha CAMK2A Rabbit Monoclonal Antibody (M03241)
Hello CJ!
M03241 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ma L,Chen X,Zhao B,Shi Y,Han F.Enhanced apoptosis and decreased ampa receptors are involved in deficit in fear memory in rin1 knockout rats.J Affect Disord.2020 May 1;268:173-182.doi:10.1016/j.jad.2020.02.040. Epub 2020 Feb 27.PMID:32174475.
Species: Rat
Inhibition of CaMKII%u03B1 in the central nucleus of amygdala attenuates fentanyl-induced hyperalgesia in rats
Anti-Epileptic Effect of Ganoderma Lucidum Polysaccharides by Inhibition of Intracellular Calcium Accumulation and Stimulation of Expression of CaMKII ? in Epileptic Hippocampal Neurons
Li Z, Yin P, Chen J, Jin S, Liu J, Luo F. PLoS One. 2017 May 10;12(5):e0177412. doi: 10.1371/journal.pone.0177412. eCollection 2017. CaMKIIα may modulate fentanyl-induced hyperalgesia via a CeLC-PAG-RVM-spinal cord descending facilitative pain pat...
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