Anti-CaMKII alpha CAMK2A Rabbit Monoclonal Antibody

Product Name

Anti-CaMKII alpha CAMK2A Rabbit Monoclonal Antibody

View all CAMK2A Antibodies

SKU/Catalog Number

M03241

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-CaMKII alpha CAMK2A Rabbit Monoclonal Antibody catalog # M03241. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CaMKII alpha CAMK2A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03241)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AAFF-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human CaMKII alpha

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M03241 is reactive to CAMK2A in Human, Mouse, Rat

Applications

M03241 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee

Observed Molecular Weight

54 kDa

Calculated molecular weight

54088 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IP 1:50
FC 1:100

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of DBN1 using anti-DBN1 antibody (M05530).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DBN1 antigen affinity purified monoclonal antibody (Catalog # M05530) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DBN1 at approximately 120 kDa. The expected band size for DBN1 is at 71 kDa.

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Western blot analysis of CaMKII alpha expression in SH-SY5Y cell lysate.

Gene/Protein Information For CAMK2A (Source: Uniprot.org, NCBI)

Alternative Names

calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase II alpha-B subunit; CaM kinase II alpha subunit; CaM Kinase II alpha; CaM kinase II subunit alpha; CAMK2A; CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha; CaMK-II alpha subunit; CaMK-II subunit alpha; CaMKIINalpha; CaM-kinase II alpha chain; EC 2.7.11; EC 2.7.11.17; KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain CAMK2A CAMKA, CaMKIINalpha, CaMKIIalpha, MRD53, MRT63 calcium/calmodulin dependent protein kinase II alpha calcium/calmodulin-dependent protein kinase type II subunit alpha|CaM kinase II alpha subunit|CaM-kinase II alpha chain|CaMK-II alpha subunit|caM kinase II subunit alpha|caMK-II subunit alpha|calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha|calcium/calmodulin-dependent protein kinase II alpha-B subunit|calcium/calmodulin-dependent protein kinase type II alpha chain

For more info on CAMK2A, check out the CAMK2A Infographic

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In this infographic, you will see the following information for CAMK2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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