Anti-CAD Dffb Antibody

Product Name

Anti-CAD Dffb Antibody

View all DFF40/CAD Antibodies

SKU/Catalog Number

A05374-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CAD Dffb Antibody (Catalog # A05374-2). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Mouse, Rat.

Storage & Handling

CAD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-CAD Dffb Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05374-2)

Host

Rabbit

Contents

CAD Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

CAD antibody was raised against a peptide corresponding to 17 amino acids near the center of murine CAD. The immunogen is located within amino acids 190 - 240 of CAD.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Multiple isoforms of GLS2 are known to exist.

Reactive Species

A05374-2 is reactive to DFFB in Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

39449 MW

Background of DFF40/CAD

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05374-2 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CAD antibody can be used for detection of CAD by Western blot at 2 μg/mL. A 40 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For DFFB (Source: Uniprot.org, NCBI)

Alternative Names

CAD, CPAN, 40kDa, DFF40, Didff, 5730477D02Rik, Cad, DNA fragmentation factor subunit beta, Caspase-activated deoxyribonuclease, CAD DFFB CAD, CPAN, DFF-40, DFF2, DFF40 DNA fragmentation factor subunit beta DNA fragmentation factor subunit beta|DNA fragmentation factor 40 kDa subunit|DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)|caspase-activated DNase|caspase-activated deoxyribonuclease|caspase-activated nuclease

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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