Product Info Summary
SKU: | PA1463 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Lipoamide Dehydrogenase/DLD Antibody
View all Dihydrolipoamide Dehydrogenase/DLD Antibodies
SKU/Catalog Number
PA1463
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Lipoamide Dehydrogenase/DLD Antibody catalog # PA1463. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lipoamide Dehydrogenase/DLD Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1463)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human DLD, different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1463 is reactive to DLD in Human, Mouse, Rat
Applications
PA1463 is guaranteed for IF, IHC, IHC-F, ICC, WB Boster Guarantee
Observed Molecular Weight
54 kDa
Calculated molecular weight
54.177kDa
Background of Dihydrolipoamide Dehydrogenase/DLD
DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex (BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Mouse
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-DLD antibody, PA1463, Western blotting
Lane 1: Rat Liver Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Ovary Tissue Lysate
Lane 4: Rat Testis Tissue Lysate
Lane 5: SMMC Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: JURKAT Cell Lysate
Click image to see more details
Anti-DLD antibody, PA1463, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Click image to see more details
Anti-Lipoamide Dehydrogenase antibody, PA1463, ICC
ICC: MCF-7 Cell
Click image to see more details
Figure 4. IF analysis of DLD using anti-DLD antibody (PA1463)
DLD was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-DLD Antibody (PA1463) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. IF analysis of DLD using anti-DLD antibody (PA1463).
DLD was detected in immunocytochemical section of HCT116 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-DLD Antibody (PA1463) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For DLD (Source: Uniprot.org, NCBI)
Gene Name
DLD
Full Name
Dihydrolipoyl dehydrogenase, mitochondrial
Weight
54.177kDa
Superfamily
class-I pyridine nucleotide-disulfide oxidoreductase family
Alternative Names
2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex); branched chain keto acid dehydrogenase complex; Diaphorase; dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex; Dihydrolipoamide Dehydrogenase; dihydrolipoamide dehydrogenasemitochondrial; DLD; DLDD; DLDH; E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex; E3; EC 1.8.1; EC 1.8.1.4; GCSL; Glycine cleavage system L protein; glycine cleavage system protein L; LAD; Lipoamide Dehydrogenase; lipoamide reductase; lipoyl dehydrogenase; PHE3 DLD DLDDH, E3, GCSL, LAD, OGDC-E3, PHE3, DLD dihydrolipoamide dehydrogenase dihydrolipoyl dehydrogenase, mitochondrial|E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex|diaphorase|epididymis secretory sperm binding protein|glycine cleavage system L protein|glycine cleavage system protein L|lipoamide dehydrogenase|lipoamide reductase|lipoyl dehydrogenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DLD, check out the DLD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DLD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lipoamide Dehydrogenase/DLD Antibody (PA1463)
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No publications found for PA1463
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