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Key=meta_description, value=Boster Bio Anti-Lipoamide Dehydrogenase/DLD Antibody catalog # PA1463. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody.
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Key=size, value=100 μg/vial
Key=protein_name, value=Dihydrolipoyl dehydrogenase, mitochondrial
Key=gene_full_name, value=Dihydrolipoyl dehydrogenase, mitochondrial
Key=uniprot_id, value=P09622
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Key=applications, value=IF, IHC, IHC-F, ICC, WB
Key=reactivity, value=Human, Mouse, Rat
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
Key=source_company, value=Boster PA1463
Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=54177 MW
Key=sample_size_available, value=30ug for $99, contact us for details
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Key=description, value=Boster Bio Anti-Lipoamide Dehydrogenase/DLD Antibody catalog # PA1463. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-Lipoamide Dehydrogenase/DLD Antibody catalog # PA1463. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-Lipoamide Dehydrogenase/DLD Antibody
Key=contents, value=Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg Thimerosal, 0.01mg NaN3.
Key=background, value=DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex (BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.
Key=gene_name, value=DLD
Key=synonyms, value=Dihydrolipoyl dehydrogenase, mitochondrial;1.8.1.4;Dihydrolipoamide dehydrogenase;Glycine cleavage system L protein;DLD;GCSL, LAD, PHE3;
Key=application_details, value=Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Mouse
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human

Key=subcellular_localization, value= Mitochondrion matrix.
Key=sequence_similarities, value=Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
Key=tissue_specificity, value= Placenta.
Key=protein_function, value= Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
Key=application_notes, value=Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 55932|sc 376890|sc 271569|sc 135027|sc 365977
Key=immunogen, value=A synthetic peptide corresponding to a sequence at the C-terminus of human DLD, different from the related mouse and rat sequences by one amino acid.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=54 kDa
Key=custom_attribute_2, value=WB: human K562 whole cell, human A431 whole cell, human SiHa whole cell, human U251 whole cell, rat liver tissue, rat heart tissue, mouse liver tissue, mouse heart tissue
IHC: Human Mammary Cancer Tissue
ICC/IF: HCT116 cell
ICC: MCF-7 Cell
IF: rat liver tissue
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Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband®

Dihydrolipoamide Dehydrogenase/DLD antibody

Boster Bio Anti-Lipoamide Dehydrogenase/DLD Antibody catalog # PA1463. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PA1463
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, IHC-F, ICC, WB

Product Name

Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband®

View all Dihydrolipoamide Dehydrogenase/DLD Antibodies

SKU/Catalog Number

PA1463

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Lipoamide Dehydrogenase/DLD Antibody catalog # PA1463. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1463)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg Thimerosal, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human DLD, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1463 is reactive to DLD in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

54 kDa

Calculated molecular weight

54177 MW

Background of Dihydrolipoamide Dehydrogenase/DLD

DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex (BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA1463 is guaranteed for IF, IHC, IHC-F, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Mouse
Immunocytochemistry , 0.5-1μg/ml, Human, -
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human

Positive Control

WB: human K562 whole cell, human A431 whole cell, human SiHa whole cell, human U251 whole cell, rat liver tissue, rat heart tissue, mouse liver tissue, mouse heart tissue
IHC: Human Mammary Cancer Tissue
ICC/IF: HCT116 cell
ICC: MCF-7 Cell
IF: rat liver tissue

Validation Images & Assay Conditions

Gene/Protein Information For DLD (Source: Uniprot.org, NCBI)

Gene Name

DLD

Full Name

Dihydrolipoyl dehydrogenase, mitochondrial

Weight

54177 MW

Superfamily

class-I pyridine nucleotide-disulfide oxidoreductase family

Alternative Names

Dihydrolipoyl dehydrogenase, mitochondrial;1.8.1.4;Dihydrolipoamide dehydrogenase;Glycine cleavage system L protein;DLD;GCSL, LAD, PHE3; DLD DLDDH, E3, GCSL, LAD, OGDC-E3, PHE3, DLD dihydrolipoamide dehydrogenase dihydrolipoyl dehydrogenase, mitochondrial|E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex|diaphorase|epididymis secretory sperm binding protein|glycine cleavage system L protein|glycine cleavage system protein L|lipoamide dehydrogenase|lipoamide reductase|lipoyl dehydrogenase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DLD, check out the DLD Infographic

DLD infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DLD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
PA1463

100μg

$370
PA1463-10ug

10μg sample (liquid)

$99
PA1463-Biotin

100 μg Biotin conjugated

$570
PA1463-Cy3

100 μg Cy3 conjugated

$570
PA1463-Dylight488

100 μg Dylight488 conjugated

$570
PA1463-Dylight550

100 μg Dylight550 conjugated

$570
PA1463-Dylight594

100 μg Dylight594 conjugated

$570
PA1463-FITC

100 μg FITC conjugated

$570
PA1463-HRP

100 μg HRP conjugated

$570
PA1463-APC

100 μg APC conjugated

$670
PA1463-PE

100 μg PE conjugated

$670
PA1463-iFluor647

100 μg iFluor647 conjugated

$670
PA1463-carrier-free

Carrier Free

$370

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PA1463
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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