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Key=created_at, value=2015-08-24 14:46:31
Key=updated_at, value=2024-09-26 00:46:40
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Key=name, value=Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband®
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Key=host, value=Rabbit
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Key=price, value=370.0000
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Key=meta_title, value=Lipoamide Dehydrogenase/DLD Antibody
Key=meta_description, value=Boster Bio Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband® catalog # PB9579. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody.
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Key=image_label, value=Anti- DLD Picoband antibody, PB9579, Western blotting
All lanes: Anti DLD (PB9579) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Rat Testis Tissue Lysate at 50ug
Lane 4: Mouse Ovary
Key=small_image_label, value=Anti- DLD Picoband antibody, PB9579, Western blotting
All lanes: Anti DLD (PB9579) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Rat Testis Tissue Lysate at 50ug
Lane 4: Mouse Ovary
Key=thumbnail_label, value=Anti- DLD Picoband antibody, PB9579, Western blotting
All lanes: Anti DLD (PB9579) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Rat Testis Tissue Lysate at 50ug
Lane 4: Mouse Ovary
Key=msrp_display_actual_price_type, value=
Key=gift_message_available, value=No
Key=size, value=100 μg/vial
Key=protein_name, value=Dihydrolipoyl dehydrogenase, mitochondrial
Key=gene_full_name, value=Dihydrolipoyl dehydrogenase, mitochondrial
Key=uniprot_id, value=P09622
Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Key=applications, value=Flow Cytometry, IF, IHC, ICC, WB
Key=reactivity, value=Human, Mouse, Rat
Key=form, value=Lyophilized
Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Key=cross_reactivity, value=No cross-reactivity with other proteins
Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
Key=source_company, value=Boster PB9579
Key=isotype, value=Rabbit IgG
Key=molecular_weight, value=54177 MW
Key=sample_size_available, value=30ug for $99, contact us for details
Key=related_product_skus, value=B0001-5,AR1156,AR0146
Key=product_category, value=Primary Antibodies
Key=research_category, value=Cell Biology, Cell Cycle, Cell Differentiation, Developmental Biology, Embryogenesis, Lineage Specification, Nervous System Development, Neurogenesis, Neurology Process, Neuroscience, Organogenesis
Key=clonality, value=Polyclonal
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Key=cj_related_products, value=Chondrocyte Hypertrophy:PA1123^Language Disorders:PA1593|A00723^Sulfate Reduction:PA1463|M00870
Key=description, value=Boster Bio Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband® catalog # PB9579. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=short_description, value=Boster Bio Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband® catalog # PB9579. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key=meta_keyword, value=Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband®
Key=contents, value=Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Key=background, value=DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex (BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.
Key=gene_name, value=DLD
Key=synonyms, value=Dihydrolipoyl dehydrogenase, mitochondrial;1.8.1.4;Dihydrolipoamide dehydrogenase;Glycine cleavage system L protein;DLD;GCSL, LAD, PHE3;
Key=application_details, value=Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Key=subcellular_localization, value= Mitochondrion matrix.
Key=sequence_similarities, value=Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
Key=tissue_specificity, value= Expressed in all tissues examined including fetal brain, fibroblasts, heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Highest levels in heart and skeletal muscle. .
Key=protein_function, value= Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.
Key=application_notes, value=Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
Key=competitor_equivalent_skus, value=sc 55932|sc 376890|sc 271569|sc 135027|sc 365977
Key=immunogen, value=E.coli-derived human DLD recombinant protein (Position: K300-F509). Human DLD shares 96.2% and 95.7% amino acid (aa) sequence identity with mouse and rat DLD, respectively.
Key=purification, value=Immunogen affinity purified.
Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Key=custom_attribute_1, value=54 kDa
Key=custom_attribute_2, value=WB: human K562 whole cell, human A431 whole cell, human SiHa whole cell, human U251 whole cell, rat liver tissue, rat heart tissue, mouse liver tissue, mouse heart tissue
IHC: human intestinal cancer tissue
ICC/IF: MCF-7 cell
FCM: A549 cell
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Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband®

Dihydrolipoamide Dehydrogenase/DLD antibody

Boster Bio Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband® catalog # PB9579. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: PB9579
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband®

View all Dihydrolipoamide Dehydrogenase/DLD Antibodies

SKU/Catalog Number

PB9579

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband® catalog # PB9579. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9579)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human DLD recombinant protein (Position: K300-F509). Human DLD shares 96.2% and 95.7% amino acid (aa) sequence identity with mouse and rat DLD, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9579 is reactive to DLD in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

54 kDa

Calculated molecular weight

54177 MW

Background of Dihydrolipoamide Dehydrogenase/DLD

DLD, Dihydrolipoamide dehydrogenase, is a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acid dehydrogenase complex (BCKD). DLD is a flavoprotein enzyme that degrades lipoamide, and produces dihydrolipoamide. The DLD gene contains 14 exons. The gene is localized to 7q31-q32. This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PB9579 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human

Positive Control

WB: human K562 whole cell, human A431 whole cell, human SiHa whole cell, human U251 whole cell, rat liver tissue, rat heart tissue, mouse liver tissue, mouse heart tissue
IHC: human intestinal cancer tissue
ICC/IF: MCF-7 cell
FCM: A549 cell

Validation Images & Assay Conditions

Gene/Protein Information For DLD (Source: Uniprot.org, NCBI)

Gene Name

DLD

Full Name

Dihydrolipoyl dehydrogenase, mitochondrial

Weight

54177 MW

Superfamily

class-I pyridine nucleotide-disulfide oxidoreductase family

Alternative Names

Dihydrolipoyl dehydrogenase, mitochondrial;1.8.1.4;Dihydrolipoamide dehydrogenase;Glycine cleavage system L protein;DLD;GCSL, LAD, PHE3; DLD DLDDH, E3, GCSL, LAD, OGDC-E3, PHE3, DLD dihydrolipoamide dehydrogenase dihydrolipoyl dehydrogenase, mitochondrial|E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex|diaphorase|epididymis secretory sperm binding protein|glycine cleavage system L protein|glycine cleavage system protein L|lipoamide dehydrogenase|lipoamide reductase|lipoyl dehydrogenase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on DLD, check out the DLD Infographic

DLD infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DLD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Lipoamide Dehydrogenase/DLD Antibody Picoband®

Question

We are currently using anti-Lipoamide Dehydrogenase/DLD antibody PB9579 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?

A. Parker

Verified customer

Asked: 2017-08-28

Answer

The anti-Lipoamide Dehydrogenase/DLD antibody (PB9579) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-08-28

Order DetailsPrice
PB9579

100μg

$370
PB9579-10ug

10μg sample (liquid)

$99
PB9579-Biotin

100 μg Biotin conjugated

$570
PB9579-Cy3

100 μg Cy3 conjugated

$570
PB9579-Dylight488

100 μg Dylight488 conjugated

$570
PB9579-Dylight550

100 μg Dylight550 conjugated

$570
PB9579-Dylight594

100 μg Dylight594 conjugated

$570
PB9579-FITC

100 μg FITC conjugated

$570
PB9579-HRP

100 μg HRP conjugated

$570
PB9579-APC

100 μg APC conjugated

$670
PB9579-PE

100 μg PE conjugated

$670
PB9579-iFluor647

100 μg iFluor647 conjugated

$670
PB9579-carrier-free

Carrier Free

$370

More conjugates/formats

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PB9579
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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