Complement Component C5a Mouse Recombinant Protein

Product Name

Complement Component C5a Mouse Recombinant Protein

View all C5 recombinant proteins

SKU/Catalog Number

PROTP06684

Size

2ug, 10ug, 1mg

Description

C5a Mouse Recombinant produced in E. coli is a single, non-glycosylated, Polypeptide chain containing 77 amino acids and having a molecular mass of 9kDa. The Mouse C5a is purified by proprietary chromatographic techniques.

Storage & Handling

Lyophilized Mouse C5a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse C5a should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.

Cite This Product

Complement Component C5a Mouse Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP06684)

Form

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Mouse C5a was lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 7.5 and 350mM NaCl.

Purity

Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

Predicted MW

188.305kDa

Reconstitution

It is recommended to reconstitute the lyophilized Mouse C5a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Biological Activity

The ED50 of Recombinant Mouse C5a as determined by its ability to induce N-acetyl-b-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells was 5-20ng/ml.

Reconstitution

It is recommended to reconstitute the lyophilized Mouse C5a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Validation Images & Assay Conditions

Gene/Protein Information For C5 (Source: Uniprot.org, NCBI)

Alternative Names

Complement C5; Hemolytic complement; C5; Hc; He; C5a C5 C5Da, C5b, CPAMD4, ECLZB, C5 complement C5 complement C5|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4|C5a anaphylatoxin|anaphylatoxin C5a analog|complement component 5|prepro-C5

*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".

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