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Product Info Summary
| SKU: | A10791-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-C21orf45/MIS18A Antibody Picoband®
SKU/Catalog Number
A10791-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-C21orf45/MIS18A Antibody Picoband® catalog # A10791-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-C21orf45/MIS18A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10791-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human C21orf45/MIS18A recombinant protein (Position: M1-D233).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10791-1 is reactive to MIS18A in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
26 kDa
Calculated molecular weight
29370 MW
Background of MIS18A
Protein Mis18-alpha is a protein that in humans is encoded by the MIS18A gene. C21orf45, also named as MIS18A, C21orf46 and FASP1, is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. C21orf45 has dimer (~52 kDa) and heterodimer(~105 kDa) forms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10791-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human 293T whole cell, human Hela whole cell
ICC/IF: HELA cell
FCM: HEL cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of C21orf45/MIS18A using anti-C21orf45/MIS18A antibody (A10791-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C21orf45/MIS18A antigen affinity purified polyclonal antibody (Catalog # A10791-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C21orf45/MIS18A at approximately 26 kDa. The expected band size for C21orf45/MIS18A is at 26 kDa.
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Figure 2. IF analysis of C21orf45/MIS18A using anti-C21orf45/MIS18A antibody (A10791-1) and anti-Beta Tubulin antibody (M01857-3).
C21orf45/MIS18A was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-C21orf45/MIS18A Antibody (A10791-1) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of HEL cells using anti-C21orf45/MIS18A antibody (A10791-1).
Overlay histogram showing HEL cells stained with A10791-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C21orf45/MIS18A Antibody (A10791-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MIS18A (Source: Uniprot.org, NCBI)
Gene Name
MIS18A
Full Name
Protein Mis18-alpha
Weight
29370 MW
Superfamily
mis18 family
Alternative Names
RNA-binding protein Nova-2; Astrocytic NOVA1-like RNA-binding protein; Neuro-oncological ventral antigen 2; NOVA2; ANOVA; NOVA3 MIS18A B28, C21orf45, C21orf46, FASP1, MIS18alpha, hMis18alpha MIS18 kinetochore protein A protein Mis18-alpha|FAPP1-associated protein 1|MIS18 kinetochore protein homolog A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MIS18A, check out the MIS18A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MIS18A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-C21orf45/MIS18A Antibody Picoband® (A10791-1)
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