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Product Info Summary
| SKU: | A10416-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-C20orf74/RALGAPA2 Antibody Picoband®
SKU/Catalog Number
A10416-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-C20orf74/RALGAPA2 Antibody Picoband® catalog # A10416-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-C20orf74/RALGAPA2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10416-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human C20orf74/RALGAPA2 recombinant protein (Position: R246-Q1556).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10416-1 is reactive to RALGAPA2 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
250 kDa
Calculated molecular weight
41409 MW
Background of RALGAPA2
Predicted to enable GTPase activator activity and protein heterodimerization activity. Predicted to be involved in activation of GTPase activity. Predicted to act upstream of or within Ral protein signal transduction; regulation of exocyst localization; and regulation of protein localization. Located in cytosol and plasma membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10416-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human RT4 whole cell
FCM: HepG2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of C20orf74/RALGAPA2 using anti-C20orf74/RALGAPA2 antibody (A10416-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C20orf74/RALGAPA2 antigen affinity purified polyclonal antibody (Catalog # A10416-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C20orf74/RALGAPA2 at approximately 250 kDa. The expected band size for C20orf74/RALGAPA2 is at 211 kDa.
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Figure 2. Flow Cytometry analysis of HepG2 cells using anti-C20orf74/RALGAPA2 antibody (A10416-1).
Overlay histogram showing HepG2 cells stained with A10416-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C20orf74/RALGAPA2 Antibody (A10416-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RALGAPA2 (Source: Uniprot.org, NCBI)
Gene Name
RALGAPA2
Full Name
Ral GTPase-activating protein subunit alpha-2
Weight
41409 MW
Alternative Names
E3 ubiquitin-protein ligase RNF169; RING finger protein 169; RING-type E3 ubiquitin transferase RNF169; RNF169; KIAA1991 RALGAPA2 AS250, C20orf74, bA287B20.1, dJ1049G11, dJ1049G11.4, p220 Ral GTPase activating protein catalytic subunit alpha 2 ral GTPase-activating protein subunit alpha-2|250 kDa substrate of Akt|Ral GTPase activating protein catalytic alpha subunit 2|Ral GTPase activating protein, alpha subunit 2 (catalytic)|RapGAPalpha2|akt substrate AS250|ral GTPase-activating protein alpha subunit 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RALGAPA2, check out the RALGAPA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RALGAPA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-C20orf74/RALGAPA2 Antibody Picoband® (A10416-1)
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