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Product Info Summary
| SKU: | A10416 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-AS250 RALGAPA2 Antibody
SKU/Catalog Number
A10416
Size
100ul
Form
Liquid
Description
Boster Bio Anti-AS250 RALGAPA2 Antibody (Catalog# A10416). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AS250 RALGAPA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10416)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human AS250. Immunogen sequence location: 641-690
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10416 is reactive to RALGAPA2 in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
210770 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10416 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from Jurkat cells, using AS250 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using AS250 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of HeLa cells, using AS250 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For RALGAPA2 (Source: Uniprot.org, NCBI)
Gene Name
RALGAPA2
Full Name
Ral GTPase-activating protein subunit alpha-2
Weight
210770 MW
Alternative Names
RALGAPA2;C20orf74;KIAA1272;Ral GTPase-activating protein subunit alpha-2;250 kDa substrate of Akt;AS250;p220 RALGAPA2 AS250, C20orf74, bA287B20.1, dJ1049G11, dJ1049G11.4, p220 Ral GTPase activating protein catalytic subunit alpha 2 ral GTPase-activating protein subunit alpha-2|250 kDa substrate of Akt|Ral GTPase activating protein catalytic alpha subunit 2|Ral GTPase activating protein, alpha subunit 2 (catalytic)|RapGAPalpha2|akt substrate AS250|ral GTPase-activating protein alpha subunit 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RALGAPA2, check out the RALGAPA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RALGAPA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AS250 RALGAPA2 Antibody (A10416)
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Customer Q&As
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3 Customer Q&As for Anti-AS250 RALGAPA2 Antibody
Question
We are currently using anti-AS250 antibody A10416 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
The anti-AS250 antibody (A10416) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-20
Question
you antibody to test anti-AS250 antibody A10416 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-AS250 antibody A10416 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-12
Answer
The products we sell, including anti-AS250 antibody A10416, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-12
Question
I was wanting to use your anti-AS250 antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
C. Roberts
Verified customer
Asked: 2015-07-20
Answer
It shows on the product datasheet, A10416 anti-AS250 antibody has been validated for IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-07-20
