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Product Info Summary
| SKU: | M00297-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-C-FOS Monoclonal Antibody
SKU/Catalog Number
M00297-3
Size
100ul
Description
Boster Bio Anti-C-FOS Monoclonal Antibody catalog # M00297-3. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-C-FOS Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00297-3)
Host
Mouse
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Monoclonal
Immunogen
Full length recombinant human protein expressed in and purified from E. coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00297-3 is reactive to FOS in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
40695 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00297-3 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-2,000, IF/ICC or IHC: 1:1,000
Validation Images & Assay Conditions
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Western blot analysis of cell lysates using mouse mAb to cFos, M00297-3, dilution 1:1,000, in green, and rabbit pmAb to GAPDH, dilution 1:20,000, in red, used as a loading control. [1] protein standard (red), [2] HeLa cells in serum free media. [3] HeLa cells stimulated with 20% fetal bovine serum for 2hrs after 36hrs in serum free media. [4] rat cortical neurons. [5] rat cortical neurons treated with membrane depolarization buffer for 5hrs. Multiple bands at 50-65kDa in stimulated or treated cell lysates correspond to different forms of the c-Fos proten. The single band at 37 kDa represents GAPDH protein.
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Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS M00297-3 in red and counterstained with rabbit polyclonal antibody to FOX3/NeuN . DAPI reveals nuclei of neurons and glia in blue. The hippocampal neurons stain green for FOX3/NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed.
Protein Target Info & Infographic
Gene/Protein Information For FOS (Source: Uniprot.org, NCBI)
Gene Name
FOS
Full Name
Proto-oncogene c-Fos
Weight
40695 MW
Superfamily
bZIP family
Alternative Names
Proto-oncogene c-Fos;Cellular oncogene fos;G0/G1 switch regulatory protein 7;FOS;G0S7; FOS AP-1, C-FOS, p55 Fos proto-oncogene, AP-1 transcription factor subunit proto-oncogene c-Fos|FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)|FBJ murine osteosarcoma viral oncogene homolog|Fos proto-oncogene, AP-1 trancription factor subunit|G0/G1 switch regulatory protein 7|activator protein 1|cellular oncogene c-fos
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FOS, check out the FOS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FOS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-C-FOS Monoclonal Antibody (M00297-3)
Hello CJ!
M00297-3 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Neurotoxicity of intrathecal injections of dexmedetomidine into the rat spinal dorsal horn
Positional and expressive alteration of prohibitin during the induced differentiation of human hepatocarcinoma SMMC-7721 cells
miR-144 and targets, c-fos and cyclooxygenase-2 (COX2), modulate synthesis of PGE2 in the amnion during pregnancy and labor
Protective effect of Angelica sinensis on cerebral neurons from rat embryos under hypoxia**
Effects of acute and chronic administration of MK-801 on c-Fos protein expression in mice brain regions implicated in schizophrenia with or without clozapine
Correlation between the Expression of c-Fos and Osteoclasts in Induced Periapical Lesions in Rats
Overexpression of c-fos in Helicobacter pylori-induced gastric precancerosis of Mongolian gerbil
Changes in the expression of c-fos & heat shock protein genes & blood flow velocity in the brain of rats undergoing myocardial ischaemia/reperfusion
Analgesic Neural Circuits Are Activated by Electroacupuncture at Two Sets of Acupoints
miR-144 and targets, c-fos and cyclooxygenase-2 (COX2), modulate synthesis of PGE2 in the amnion during pregnancy and labor
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Customer Q&As
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15 Customer Q&As for Anti-C-FOS Monoclonal Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mucosa of stomach using anti-C-FOS Monoclonal antibody M00297-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-25
Question
Here is the WB image, lot number and protocol we used for mucosa of stomach using anti-C-FOS Monoclonal antibody M00297-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-03-25
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-25
Question
We purchased anti-C-FOS Monoclonal antibody for WB on mucosa of stomach in a previous experiment. I am using mouse, and We intend to use the antibody for IF next. My question regards examining mucosa of stomach as well as leukemic t-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-03-01
Answer
I looked at the website and datasheets of our anti-C-FOS Monoclonal antibody and it seems that M00297-3 has been tested on mouse in both WB and IF. Thus M00297-3 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-03-01
Question
Does M00297-3 anti-C-FOS Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
It shows on the product datasheet, M00297-3 anti-C-FOS Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-15
Question
I see that the anti-C-FOS Monoclonal antibody M00297-3 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-28
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-03-28
Question
Is this M00297-3 anti-C-FOS Monoclonal antibody reactive to the isotypes of FOS?
Verified Customer
Verified customer
Asked: 2018-02-28
Answer
The immunogen of M00297-3 anti-C-FOS Monoclonal antibody is Full length recombinant human protein expressed in and purified from E. coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-02-28
Question
I was wanting to use your anti-C-FOS Monoclonal antibody for WB for mouse mucosa of stomach on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse mucosa of stomach identification?
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
You can see on the product datasheet, M00297-3 anti-C-FOS Monoclonal antibody has been validated for IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse mucosa of stomach in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-06
Question
We are currently using anti-C-FOS Monoclonal antibody M00297-3 for rat tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-11
Answer
The anti-C-FOS Monoclonal antibody (M00297-3) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-11
Question
Is a blocking peptide available for product anti-C-FOS Monoclonal antibody (M00297-3)?
Verified Customer
Verified customer
Asked: 2017-09-01
Answer
We do provide the blocking peptide for product anti-C-FOS Monoclonal antibody (M00297-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-01
Question
My team were content with the WB result of your anti-C-FOS Monoclonal antibody. However we have observed positive staining in mucosa of stomach nucleus. endoplasmic reticulum. cytoplasm, using this antibody. Is that expected? Could you tell me where is FOS supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-08-21
Answer
According to literature, mucosa of stomach does express FOS. Generally FOS expresses in nucleus. endoplasmic reticulum. cytoplasm,. Regarding which tissues have FOS expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-08-21
Question
I am interested in to test anti-C-FOS Monoclonal antibody M00297-3 on mouse mucosa of stomach for research purposes, then I may be interested in using anti-C-FOS Monoclonal antibody M00297-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-29
Answer
The products we sell, including anti-C-FOS Monoclonal antibody M00297-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-29
Question
Would anti-C-FOS Monoclonal antibody M00297-3 work for WB with mucosa of stomach?
J. Patel
Verified customer
Asked: 2017-05-29
Answer
According to the expression profile of mucosa of stomach, FOS is highly expressed in mucosa of stomach. So, it is likely that anti-C-FOS Monoclonal antibody M00297-3 will work for WB with mucosa of stomach.
Boster Scientific Support
Answered: 2017-05-29
Question
We have observed staining in human leukemic t-cell. Are there any suggestions? Is anti-C-FOS Monoclonal antibody supposed to stain leukemic t-cell positively?
Z. Yang
Verified customer
Asked: 2015-07-28
Answer
According to literature leukemic t-cell does express FOS. According to Uniprot.org, FOS is expressed in mucosa of stomach, colon, pancreas, leukemic t-cell, among other tissues. Regarding which tissues have FOS expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 14702039
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2015-07-28
Question
Can you help my question with product M00297-3, anti-C-FOS Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Li
Verified customer
Asked: 2015-03-31
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00297-3 anti-C-FOS Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-03-31
Question
Do you have a BSA free version of anti-C-FOS Monoclonal antibody M00297-3 available?
J. Jha
Verified customer
Asked: 2013-03-27
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-C-FOS Monoclonal antibody M00297-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-03-27
