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Product Info Summary
| SKU: | A03118-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Bub3 Antibody
SKU/Catalog Number
A03118-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Bub3 Antibody catalog # A03118-1. Tested in WB,ICC,IF,IP,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bub3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03118-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant protein corresponding to the N-terminus of human Bub3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03118-1 is reactive to BUB3 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
37155 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03118-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
IP: 1:10-1:50
FC: 1:50-1:100
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of BUB3 using Anti-Bub3 Antibody (A03118-1).
ICC staining Bub3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-Bub3 Antibody A03118-1
Western blot analysis of Bub3 on different lysates using anti-Bub3 antibody at 1/500 dilution.Positive control:
Lane 1: A549
Lane 2: HL-60
Lane 3: A431
Lane 4: Rat colon
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For BUB3 (Source: Uniprot.org, NCBI)
Gene Name
BUB3
Full Name
Mitotic checkpoint protein BUB3
Weight
37155 MW
Superfamily
WD repeat BUB3 family
Alternative Names
Mitotic checkpoint protein BUB3;BUB3; BUB3 BUB3L, hBUB3 BUB3 mitotic checkpoint protein mitotic checkpoint protein BUB3|BUB3 budding uninhibited by benzimidazoles 3 homolog|budding uninhibited by benomyl|budding uninhibited by benzimidazoles 3 homolog|mitotic checkpoint component|testicular tissue protein Li 27
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BUB3, check out the BUB3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BUB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bub3 Antibody (A03118-1)
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