Anti-BRSK1 Antibody

Product Name

Anti-BRSK1 Antibody

View all BRSK1 Antibodies

SKU/Catalog Number

A05997-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-BRSK1 Antibody (Catalog # A05997-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

BRSK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-BRSK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05997-1)

Host

Rabbit

Contents

BRSK1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

BRSK1 antibody was raised against a 28 amino acid synthetic peptide from near the carboxy terminus of human BRSK1. The immunogen is located within amino acids 720 - 770 of BRSK1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

PMEPA1 antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect all of the isoforms.

Reactive Species

A05997-1 is reactive to BRSK1 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

85087 MW

Background of BRSK1

BRSK1 was initially identified as a mammalian homolog to the fission yeast S. pombe Cdr2, a mitosis-regulatory kinase and also shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK1 is unbiquitously expressed, with highest levels of expression in the brain and testes. Similar to its yeast homolog, BRSK1 is thought to be involved in stress-induced cell cycle arrest. Overexpression of this protein leads to the G2/M arrest in HeLa S2 cells and UV-induced G2/M arrest could be partially abrogated by reduced expression of BRSK1 through the use of siRNA, indicating its role in DNA damage checkpoint function. More recently, it has been shown that both BRSK1 and the related protein BRSK2 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. At least two isoforms of BRSK1 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05997-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

BRSK1 antibody can be used for detection of BRSK1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For BRSK1 (Source: Uniprot.org, NCBI)

Alternative Names

hSAD1, KIAA1811, SAD1, SADB, Brain-selective kinase 1, SAD1 homolog BRSK1 hSAD1 BR serine/threonine kinase 1 serine/threonine-protein kinase BRSK1|BR serine/threonine-protein kinase 1|SAD1 homolog|SAD1 kinase|brain-selective kinase 1|brain-specific serine/threonine-protein kinase 1|protein kinase SAD1A|serine/threonine-protein kinase SAD-B|synapses of Amphids Defective homolog 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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