Anti-BRSK1 Antibody

Product Name

Anti-BRSK1 Antibody

View all BRSK1 Antibodies

SKU/Catalog Number

A05997

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-BRSK1 Antibody (Catalog # A05997). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BRSK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05997)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human BRSK1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05997 is reactive to BRSK1 in Human, Mouse

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Calculated molecular weight

85087 MW

Background of BRSK1

Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.

Lizcano J.M., EMBO J. 23:833-843(2004).
Lu R., J. Biol. Chem. 279:31164-31170(2004).
Nagase T., DNA Res. 8:85-95(2001).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A05997 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For BRSK1 (Source: Uniprot.org, NCBI)

Alternative Names

Serine/threonine-protein kinase BRSK1;2.7.11.1;Brain-selective kinase 1;2.7.11.26;Brain-specific serine/threonine-protein kinase 1;BR serine/threonine-protein kinase 1;Serine/threonine-protein kinase SAD-B;Synapses of Amphids Defective homolog 1;SAD1 homolog;hSAD1;BRSK1;KIAA1811, SAD1, SADB; BRSK1 hSAD1 BR serine/threonine kinase 1 serine/threonine-protein kinase BRSK1|BR serine/threonine-protein kinase 1|SAD1 homolog|SAD1 kinase|brain-selective kinase 1|brain-specific serine/threonine-protein kinase 1|protein kinase SAD1A|serine/threonine-protein kinase SAD-B|synapses of Amphids Defective homolog 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis