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Product Info Summary
| SKU: | A07721 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-BRP44L MPC1 Antibody
SKU/Catalog Number
A07721
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-BRP44L MPC1 Antibody (Catalog # A07721). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BRP44L MPC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07721)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human BRP44L.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07721 is reactive to MPC1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
12347 MW
Background of BRP44L
Mediates the uptake of pyruvate into mitochondria.
Zhang Q.-H., Genome Res. 10:1546-1560(2000).
Lai C.-H., Genome Res. 10:703-713(2000).
Mungall A.J., Nature 425:805-811(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07721 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human heart tissue using BRP44L antibody A07721.
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Western blot analysis of extracts from COLO cells and HeLa cells, using BRP44L antibody A07721.
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Immunofluorescence analysis of A549 cells, using BRP44L antibody A07721.
Protein Target Info & Infographic
Gene/Protein Information For MPC1 (Source: Uniprot.org, NCBI)
Gene Name
MPC1
Full Name
Mitochondrial pyruvate carrier 1
Weight
12347 MW
Superfamily
mitochondrial pyruvate carrier (MPC) (TC 2.A.105) family
Alternative Names
Mitochondrial pyruvate carrier 1;Brain protein 44-like protein;MPC1;BRP44L;CGI-129, HSPC040, PNAS-115; MPC1 BRP44L, CGI-129, MPYCD, SLC54A1 mitochondrial pyruvate carrier 1 mitochondrial pyruvate carrier 1|HSPC040 protein|brain protein 44-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MPC1, check out the MPC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MPC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BRP44L MPC1 Antibody (A07721)
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No publications found for A07721
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Customer Q&As
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3 Customer Q&As for Anti-BRP44L MPC1 Antibody
Question
I have a question about product A07721, anti-BRP44L antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07721 anti-BRP44L antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-27
Question
Would A07721 anti-BRP44L antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
As indicated on the product datasheet, A07721 anti-BRP44L antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-19
Question
We need to test anti-BRP44L antibody A07721 on human liver for research purposes, then I may be interested in using anti-BRP44L antibody A07721 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-12-01
Answer
The products we sell, including anti-BRP44L antibody A07721, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-12-01
