Anti-MED23/Crsp130 Antibody

Product Name

Anti-MED23/Crsp130 Antibody

View all MED23 Antibodies

SKU/Catalog Number

A03859

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MED23/Crsp130 Antibody (Catalog # A03859). Tested in WB, IHC, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MED23/Crsp130 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03859)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MED23.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03859 is reactive to MED23 in Human

Reconstitution

Calculated molecular weight

156474 MW

Background of MED23

Required for transcriptional activation subsequent to the assembly of the preinitiation complex By similarity. Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.

Wiemann S., Genome Res. 11:422-435(2001).
Mungall A.J., Nature 425:805-811(2003).
Akoulitchev S., Nature 407:102-106(2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03859 is guaranteed for IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For MED23 (Source: Uniprot.org, NCBI)

Alternative Names

Mediator of RNA polymerase II transcription subunit 23;Activator-recruited cofactor 130 kDa component;ARC130;Cofactor required for Sp1 transcriptional activation subunit 3;CRSP complex subunit 3;Mediator complex subunit 23;Protein sur-2 homolog;hSur-2;Transcriptional coactivator CRSP130;Vitamin D3 receptor-interacting protein complex 130 kDa component;DRIP130;MED23;ARC130, CRSP3, DRIP130, KIAA1216, SUR2; MED23 ARC130, CRSP130, CRSP133, CRSP3, DRIP130, MRT18, SUR-2, SUR2 mediator complex subunit 23 mediator of RNA polymerase II transcription subunit 23|130 kDa transcriptional co-activator|133 kDa transcriptional co-activator|activator-recruited cofactor 130 kDa component|cofactor required for Sp1 transcriptional activation subunit 3|mental retardation, non-syndromic, autosomal recessive, 18|vitamin D3 receptor interacting protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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