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Product Info Summary
| SKU: | M00123 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Brd4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00123
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Brd4 Rabbit Monoclonal Antibody catalog # M00123. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Brd4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00123)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DBH-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Brd4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00123 is reactive to BRD4 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa
Calculated molecular weight
152219 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00123 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
IHC: human kidney tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human kidney, using Brd4 Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of Hela cells, using Brd4 Antibody .
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Western blot analysis of Brd4 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For BRD4 (Source: Uniprot.org, NCBI)
Gene Name
BRD4
Full Name
Bromodomain-containing protein 4
Weight
152219 MW
Alternative Names
Bromodomain-containing protein 4;Protein HUNK1;BRD4;HUNK1; BRD4 CAP, HUNK1, HUNKI, MCAP bromodomain containing 4 bromodomain-containing protein 4|chromosome-associated protein|mitotic chromosome-associated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRD4, check out the BRD4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Brd4 Rabbit Monoclonal Antibody (M00123)
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Customer Q&As
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7 Customer Q&As for Anti-Brd4 Rabbit Monoclonal Antibody
Question
Will anti-Brd4 Rabbit Monoclonal antibody M00123 work on feline IHC with liver?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
Our lab technicians have not validated anti-Brd4 Rabbit Monoclonal antibody M00123 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Brd4 Rabbit Monoclonal antibody M00123 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-16
Question
Can you help my question with product M00123, anti-Brd4 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00123 anti-Brd4 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-02
Question
We are currently using anti-Brd4 Rabbit Monoclonal antibody M00123 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
The anti-Brd4 Rabbit Monoclonal antibody (M00123) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-18
Question
Would M00123 anti-Brd4 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-12-26
Answer
It shows on the product datasheet, M00123 anti-Brd4 Rabbit Monoclonal antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-26
Question
I was wanting to use your anti-Brd4 Rabbit Monoclonal antibody for ICC for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
You can see on the product datasheet, M00123 anti-Brd4 Rabbit Monoclonal antibody has been tested for IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-25
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-Brd4 Rabbit Monoclonal antibody M00123. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-12-29
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-29
Question
Will anti-Brd4 Rabbit Monoclonal antibody M00123 work for ICC with cervix carcinoma erythroleukemia?
C. Jones
Verified customer
Asked: 2014-10-30
Answer
According to the expression profile of cervix carcinoma erythroleukemia, BRD4 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-Brd4 Rabbit Monoclonal antibody M00123 will work for ICC with cervix carcinoma erythroleukemia.
Boster Scientific Support
Answered: 2014-10-30
