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Product Info Summary
| SKU: | DZ02095 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
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Product info
Product Name
Anti-Zebrafish siah1 Antibody
SKU/Catalog Number
DZ02095
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Anti-siah1 Antibody catalog # DZ02095. This antibody reacts with Zebrafish.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Zebrafish siah1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ02095)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Reactive Species
DZ02095 is reactive to siah1 in Zebrafish
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Calculated molecular weight
31.123kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Validation Images & Assay Conditions
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Figure 1. IF analysis of Siah1 using anti-Siah1 antibody (DZ02095).
Siah1 was detected in paraffin-embedded section of five-day-old zebrafish embryos tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-Siah1 Antibody (DZ02095) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®594 Conjugated Avidin (BA1142). Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For siah1 (Source: Uniprot.org, NCBI)
Gene Name
siah1
Full Name
E3 ubiquitin-protein ligase SIAH1
Weight
31.123kDa
Superfamily
SINA (Seven in absentia) family
Alternative Names
E3 ubiquitin-protein ligase SIAH1; EC 6.3.2; EC 6.3.2.-; FLJ08065; hSIAH1; HUMSIAH; seven in absentia homolog 1 (Drosophila); Seven in absentia homolog 1; Siah-1; SIAH1A; siah-1a SIAH1 SIAH1A siah E3 ubiquitin protein ligase 1 E3 ubiquitin-protein ligase SIAH1|RING-type E3 ubiquitin transferase SIAH1|seven in absentia homolog 1|siah-1a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on siah1, check out the siah1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for siah1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Zebrafish siah1 Antibody (DZ02095)
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Customer Reviews
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1 Reviews For Anti-Zebrafish siah1 Antibody
0
Good Zebrafish-Specific Siah1 Antibody from Boster Bio--Jakub Famulski, University of Kentucky, Biology, Principal Investigator
Excellent
Source: Biocompare.com
| Application | Immunofluorescence |
|---|---|
| Sample | Zebrafish embryos 5dpf |
| Detection | Confocal Microscopy |
"Antibody labels the retina outer segments and inner nuclear layers as expected based on in situ hybridization studies. The staining is clean. A zebrafish-specific antibody for Siah1 that actually WORKS!"
Jakub Famulski
Verified customer
Submitted 2019-12-06
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