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Product Info Summary
| SKU: | M00005-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-BRCA1 Mouse Monoclonal Antibody [Clone ID: OTI5D10]
SKU/Catalog Number
M00005-1
Size
100 µl
Description
Boster Bio BRCA1 mouse monoclonal antibody, clone OTI5D10 (formerly 5D10). Catalog# M00005-1. Tested in IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-BRCA1 Mouse Monoclonal Antibody [Clone ID: OTI5D10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00005-1)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI5D10
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 1151-1473 of human BRCA1 (NP_009225) produced in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00005-1 is reactive to BRCA1 in Human
Reconstitution
Calculated molecular weight
207.721kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00005-1 is guaranteed for IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRCA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRCA1.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min) (M00005-1) (1:200)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BRCA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min
Protein Target Info & Infographic
Gene/Protein Information For BRCA1 (Source: Uniprot.org, NCBI)
Gene Name
BRCA1
Full Name
Breast cancer type 1 susceptibility protein
Weight
207.721kDa
Alternative Names
BRCA1; BRCAI; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein; breast cancer 1, early onset; breast cancer type 1 susceptibility protein; EC 6.3.2; EC 6.3.2.-; IRIS; PNCA4; PSCP; subunit 1 BRCA1 BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53 BRCA1 DNA repair associated breast cancer type 1 susceptibility protein|BRCA1/BRCA2-containing complex, subunit 1|Fanconi anemia, complementation group S|RING finger protein 53|breast and ovarian cancer susceptibility protein 1|breast cancer 1, early onset|early onset breast cancer 1|protein phosphatase 1, regulatory subunit 53
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRCA1, check out the BRCA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRCA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BRCA1 Mouse Monoclonal Antibody [Clone ID: OTI5D10] (M00005-1)
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