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Product Info Summary
| SKU: | A31984 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Brachyury TBXT Antibody
SKU/Catalog Number
A31984
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Brachyury TBXT Antibody catalog # A31984. Tested in WB,ICC/IF applications. This antibody reacts with Human,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Brachyury TBXT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31984)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
RecombinanBrachyury fusion proBrachyuryein conBrachyuryaining a sequence corresponding Brachyuryo amino acids 226-435 of human Brachyury(NP_003172.1).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A31984 is reactive to TBXT in Human, Rat
Reconstitution
Calculated molecular weight
47443 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A31984 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded mouse E14.5 embryo tissue using anti-Brachyury antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 mins. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed
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Western blot analysis of Brachyury / Bry on SH-SY-5Y lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP
Protein Target Info & Infographic
Gene/Protein Information For TBXT (Source: Uniprot.org, NCBI)
Gene Name
TBXT
Full Name
T-box transcription factor T
Weight
47443 MW
Alternative Names
T-box transcription factor T ;Brachyury protein ;Protein T;TBXT TBXT SAVA, T, TFT T-box transcription factor T T-box transcription factor T|T brachyury transcription factor|T, brachyury homolog|brachyury protein|protein T
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TBXT, check out the TBXT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TBXT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Brachyury TBXT Antibody (A31984)
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