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Product Info Summary
| SKU: | A01910 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-WD repeat-containing protein 5 WDR5 Antibody
SKU/Catalog Number
A01910
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-WD repeat-containing protein 5 WDR5 Antibody (Catalog # A01910). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
WDR5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-WD repeat-containing protein 5 WDR5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01910)
Host
Rabbit
Contents
WDR5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
WDR5 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human WDR5. The immunogen is located within the first 50 amino acids of WDR5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
WDR5 antibody is human specific. WDR5 antibody is predicted to not cross-react with other WDR family members.
Reactive Species
A01910 is reactive to WDR5 in Human
Applications
A01910 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
36588 MW
Background of WDR5
WD repeat domain 5 (WDR5) is a member of the WD repeat protein family, which is involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. WDR5, also known as BIG-3, is expressed in the developing growth plate, accelerates chondrocyte and osteoblast differentiation in vitro, and regulates osteoblast differentiation during embryonic bone development. WDR5 interacts with the pluripotency factor Oct4/POU5F1 and is required for the efficient formation of induced pluripotent stem (iPS) cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WDR5 antibody can be used for detection of WDR5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of WDR5 in 293 cell lysate with WDR5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of WDR5 in 293 cells with WDR5 antibody at 5 μg/mL.
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Immunofluorescence of WDR5 in 293 cells with WDR5 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For WDR5 (Source: Uniprot.org, NCBI)
Gene Name
WDR5
Full Name
WD repeat-containing protein 5
Weight
36588 MW
Superfamily
WD repeat WDR5/wds family
Alternative Names
BIG3; BIG-3; BMP2-induced 3-kb gene protein; SWD3; SWD3, Set1c WD40 repeat protein, homolog; WD repeat domain 5; WD repeat-containing protein 5; WDR5; WD-repeat protein 5 WDR5 BIG-3, CFAP89, SWD3 WD repeat domain 5 WD repeat-containing protein 5|BMP2-induced 3-kb gene protein|SWD3, Set1c WD40 repeat protein, homolog|cilia and flagella associated protein 89
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on WDR5, check out the WDR5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for WDR5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-WD repeat-containing protein 5 WDR5 Antibody (A01910)
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